Composite microbial preparation and application thereof in relieving depression and constipation

A technology of compounding probiotics and probiotics preparations, applied in the field of microorganisms, can solve the problems of not being able to relieve depression and constipation at the same time, unilaterally relieving mental disorders and gastrointestinal diseases, etc., so as to reduce intestinal permeability and improve intestinal permeability. Peristaltic ability, the effect of wide application prospects

Active Publication Date: 2020-10-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patented strains only have the effect of unilaterally relieving mental disorder

Method used

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  • Composite microbial preparation and application thereof in relieving depression and constipation
  • Composite microbial preparation and application thereof in relieving depression and constipation
  • Composite microbial preparation and application thereof in relieving depression and constipation

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0047] Example 1 Isolation, Screening and Identification of Probiotics

[0048] (1) Isolation and screening of probiotics:

[0049] (1) Suck 1 mL of stool samples collected from different areas (stool samples stored in 30% glycerol), after gradient dilution, spread the stool samples on MRS solid medium, and cultivate them in anaerobic workstations for 48 hours. The temperature of the oxygen workstation is 37°C;

[0050] (2) Pick out the strains with different colony morphology and conduct streaking separation and purification on MRS solid medium, and record the morphological characteristics of the bacteria in detail (Table 1);

[0051] (3) Pick the single bacteria isolated from the culture medium, transfer to MRS liquid medium, and cultivate for 48 hours in an anaerobic workstation at 37°C. The obtained strains are Gram stained and the colony morphology is recorded;

[0052] (4) Abandon the gram-negative bacteria strains and gram-positive cocci in the colony, and select gram-positive b...

Example Embodiment

[0066] Example 2: Compound probiotics can relieve symptoms of depression and anxiety

[0067] 36 sex C57BL / 6J mice, 5 weeks old, weighing 18-20g, SPF grade. After one week of adapting to the environment, the experimental mice were randomly divided into three groups according to their body weight: normal control group, depression model group, single-bacterial intervention group, and compound probiotic intervention group. Each group contained 6 mice. The growth environment conditions of the experimental mice are: ambient temperature (23±2)°C, relative humidity (50±10)%, and light mode (12h dark / 12h light). See the experimental rat grouping and feeding plan figure 1 .

[0068] The model was modeled with chronic unpredictable mild stimulus. The specific stimulus factors include: (1) fasting for 24 hours; (2) water-free for 24 hours; (3) tail clamping for 3 minutes, and clip to clamp the tail tip 1 cm; (4) restraining for 2 hours; ) Forced swimming for 10 minutes, water temperature (2...

Example Embodiment

[0079] Example 3: Compound probiotics can relieve constipation symptoms caused by chronic stress

[0080] (1) First black stool experiment

[0081] The first black stool test can be used to simulate the movement time of chyme throughout the intestine. Before starting the experiment, the rats were fasted for 12 hours, and then 0.2mL of activated carbon gum arabic solution was given to each mouse. Each mouse was placed in a storage box with absorbent paper; the time and the first time when the gastric activated carbon was finished was recorded. The time when a black stool is produced, so as to calculate the total time to discharge the first black stool. The result is Figure 4 As shown in A, the time to discharge the first black stool in the depression model group and the normal control group are 257.5 minutes and 167.8 minutes, respectively. Chronic stress causes a significant delay in stool discharge, which has a significant appearance of constipation. After supplementing with pro...

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Abstract

The invention discloses a composite microbial preparation and application thereof in relieving depression and constipation, and belongs to the technical field of microorganisms. The prepared compositeprobiotics can reduce the concentration of adrenal release promoting hormone and corticosterone in serum of mice and relieve depression and anxiety behaviors caused by chronic pressure; the compositeprobiotics can up-regulate the expression of intestinal tract Htr4 genes, so that the intestinal tract peristalsis capacity of mice is improved; the content of short-chain fatty acid in intestinal tracts can be increased, and constipation can be remarkably improved; the gene expression of intestinal tract tight junction proteins ZO-1, Occludin and Claudin-1 can be improved, the intestinal tract permeability can be reduced, and peripheral and central nervous system inflammations can be reduced; the level of 5-HTP in intestinal tracts, serum and brains can be improved, and the potential of improving sleep is achieved. The composite probiotics prepared by the invention have wide application values in the aspects of resisting depression and anxiety, relieving constipation, resisting inflammation, improving sleep and the like.

Description

technical field [0001] The present invention relates to the application of compound microbial preparations and alleviating depression and constipation, in particular to the application of Bifidobacterium breve CCFM1025, Bifidobacterium longum subspecies infantile subspecies CCFM687 and Pediococcus lactis CCFM6432 in preparing antidepressant and anxiety, relieving constipation, anti-inflammation and improving sleep The application in pharmaceuticals and functional foods belongs to the field of microbial technology. Background technique [0002] Depression is a chronic and relapsing "spiritual cold". The occurrence of depression may be related to many factors such as parting, environmental pressure, childhood trauma, disease, and genes. Its common clinical symptoms are persistent depression, social isolation, fatigue and insomnia, and negative ideas such as self-mutilation and suicide. In clinical treatment, depressed patients are usually accompanied by various gastrointestin...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23C9/123A23C13/16A23C19/032A23C19/06A23L19/00A23L29/00A61K35/745A61K35/744A61P25/24A61P5/00A61P1/00A61P1/10A61P29/00
CPCA23L33/135A23C9/1234A23C13/16A23C19/0323A23C19/062A23L19/00A23L29/065A61K35/745A61K35/744A61P25/24A61P5/00A61P1/00A61P1/10A61P29/00A23V2002/00A23V2400/413A23V2400/519A23V2400/533A61K2300/00A23V2200/322A23V2200/32A23V2200/30
Inventor 王刚田培郡邹仁英翟齐啸陆文伟杨波崔树茂张秋香毛丙永唐鑫陈卫赵建新张灏
Owner JIANGNAN UNIV
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