Composite microbial preparation and application thereof in relieving depression and constipation
A technology of compounding probiotics and probiotics preparations, applied in the field of microorganisms, can solve the problems of not being able to relieve depression and constipation at the same time, unilaterally relieving mental disorders and gastrointestinal diseases, etc., so as to reduce intestinal permeability and improve intestinal permeability. Peristaltic ability, the effect of wide application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0047] Example 1 Isolation, Screening and Identification of Probiotics
[0048] (1) Isolation and screening of probiotics:
[0049] (1) Suck 1 mL of stool samples collected from different areas (stool samples stored in 30% glycerol), after gradient dilution, spread the stool samples on MRS solid medium, and cultivate them in anaerobic workstations for 48 hours. The temperature of the oxygen workstation is 37°C;
[0050] (2) Pick out the strains with different colony morphology and conduct streaking separation and purification on MRS solid medium, and record the morphological characteristics of the bacteria in detail (Table 1);
[0051] (3) Pick the single bacteria isolated from the culture medium, transfer to MRS liquid medium, and cultivate for 48 hours in an anaerobic workstation at 37°C. The obtained strains are Gram stained and the colony morphology is recorded;
[0052] (4) Abandon the gram-negative bacteria strains and gram-positive cocci in the colony, and select gram-positive b...
Example Embodiment
[0066] Example 2: Compound probiotics can relieve symptoms of depression and anxiety
[0067] 36 sex C57BL / 6J mice, 5 weeks old, weighing 18-20g, SPF grade. After one week of adapting to the environment, the experimental mice were randomly divided into three groups according to their body weight: normal control group, depression model group, single-bacterial intervention group, and compound probiotic intervention group. Each group contained 6 mice. The growth environment conditions of the experimental mice are: ambient temperature (23±2)°C, relative humidity (50±10)%, and light mode (12h dark / 12h light). See the experimental rat grouping and feeding plan figure 1 .
[0068] The model was modeled with chronic unpredictable mild stimulus. The specific stimulus factors include: (1) fasting for 24 hours; (2) water-free for 24 hours; (3) tail clamping for 3 minutes, and clip to clamp the tail tip 1 cm; (4) restraining for 2 hours; ) Forced swimming for 10 minutes, water temperature (2...
Example Embodiment
[0079] Example 3: Compound probiotics can relieve constipation symptoms caused by chronic stress
[0080] (1) First black stool experiment
[0081] The first black stool test can be used to simulate the movement time of chyme throughout the intestine. Before starting the experiment, the rats were fasted for 12 hours, and then 0.2mL of activated carbon gum arabic solution was given to each mouse. Each mouse was placed in a storage box with absorbent paper; the time and the first time when the gastric activated carbon was finished was recorded. The time when a black stool is produced, so as to calculate the total time to discharge the first black stool. The result is Figure 4 As shown in A, the time to discharge the first black stool in the depression model group and the normal control group are 257.5 minutes and 167.8 minutes, respectively. Chronic stress causes a significant delay in stool discharge, which has a significant appearance of constipation. After supplementing with pro...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap