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Preparation method of airway mucus model

A model and mucus technology, applied in the field of preparation of airway mucus models, can solve the problems of short use time, affecting the accuracy of model data, and increasing the difference between airway mucus, etc., and achieve the effect of long use time

Inactive Publication Date: 2020-10-13
深圳市龙华区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of simple process, low cost, and non-toxicity. The mucus model has biological, physical and chemical properties matching with the human airway mucus model, and is very important in the protection mechanism of mucus, pathological analysis, drug delivery, and gene carrier therapy. The airway mucus model obtained in the patent does not add mucin, the main substance that determines the rheological characteristics of the airway mucus. At that time, the difference between it and the real airway mucus will increase, which will affect the accuracy of the data used in the model for research. Moreover, the preparation method of the model cannot preserve the ingredients and moisture inside the model for a long time, and the use time is short

Method used

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  • Preparation method of airway mucus model

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Effect test

Embodiment 1

[0027] A kind of preparation method of airway mucus model, is made up of the composition of following weight raw material:

[0028] Hydroxyethyl cellulose;

[0029] Dipalmitoylphosphatidylcholine;

[0030] serum egg;

[0031] Mucin;

[0032] Benzalkonium Bromide;

[0033] Phosphate buffer saline; the mucin is the secretory mucin Mucin5AC of the airway.

[0034] Include the following steps:

[0035] (1) Preparation of model materials: put dipalmitoylphosphatidylcholine and 16ml-30ml deionized water into a container for mixing, and then stir rapidly for 5min-10min to obtain a viscous paste;

[0036] (2) Preparation of phosphate buffer: take 35.9 g of disodium hydrogen phosphate, add water to dissolve, and dilute to 500 ml to obtain liquid A, take 2.76 g of sodium dihydrogen phosphate, add water to dissolve, and dilute to 100 ml to obtain liquid B, take the above 54.5ml-82.5ml of solution A and 5.5ml-7.5ml of solution B are mixed and shaken to obtain a phosphate buffer, whe...

Embodiment 2

[0042] A kind of preparation method of airway mucus model, is made up of the composition of following weight raw material:

[0043] Hydroxyethyl cellulose;

[0044] Dipalmitoylphosphatidylcholine;

[0045] serum egg;

[0046] Mucin;

[0047] Benzalkonium Bromide;

[0048] Phosphate buffer saline; the mucin is the secreted mucin Mucin2 of the airway.

[0049] Include the following steps:

[0050] (1) Preparation of model materials: put dipalmitoylphosphatidylcholine and 16ml-30ml deionized water into a container for mixing, and then stir rapidly for 5min-10min to obtain a viscous paste;

[0051] (2) Preparation of phosphate buffer: take 35.9 g of disodium hydrogen phosphate, add water to dissolve, and dilute to 500 ml to obtain liquid A, take 2.76 g of sodium dihydrogen phosphate, add water to dissolve, and dilute to 100 ml to obtain liquid B, take the above 54.5ml-82.5ml of solution A and 5.5ml-7.5ml of solution B are mixed and shaken to obtain a phosphate buffer, wherei...

Embodiment 3

[0057] A kind of preparation method of airway mucus model, is made up of the composition of following weight raw material:

[0058] Hydroxyethyl cellulose;

[0059] Dipalmitoylphosphatidylcholine;

[0060] serum egg;

[0061] Mucin;

[0062] Benzalkonium bromide;

[0063] Phosphate buffer saline; the mucin is the secretory mucin Mucin5B of the airway.

[0064] Include the following steps:

[0065] (1) Preparation of model materials: put dipalmitoylphosphatidylcholine and 16ml-30ml deionized water into a container for mixing, and then stir rapidly for 5min-10min to obtain a viscous paste;

[0066] (2) Preparation of phosphate buffer: take 35.9 g of disodium hydrogen phosphate, add water to dissolve, and dilute to 500 ml to obtain liquid A, take 2.76 g of sodium dihydrogen phosphate, add water to dissolve, and dilute to 100 ml to obtain liquid B, take the above 54.5ml-82.5ml of solution A and 5.5ml-7.5ml of solution B are mixed and shaken to obtain a phosphate buffer, wher...

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Abstract

The invention belongs to the field of airway mucus models, and relates to a preparation method of an airway mucus model. The airway mucus model is prepared from the following raw materials: hydroxyethyl cellulose; dipalmitoyl phosphatidylcholine, serum egg, mucoprotein, benzalkonium bromide and a phosphate buffer solution; the mucoprotein is secretory mucoprotein Mucin5AC, Mucin2 or Mucin5B of anairway. The airway mucus model is prepared by a plurality of steps of preparation of model materials, preparation of the phosphate buffer solution, preparation of the model, disinfection treatment, characteristics endowment of the model and the shaping of the model. According to the preparation method of the airway mucus model, the most important mucoprotein element such as human airway mucus is added in the preparation of the airway mucus model; and benzalkonium bromide disinfection and hydroxyethyl cellulose shaping are carried out, so that the prepared airway mucus model is more suitable for the real human airway mucus, is durable and can facilitate subsequent medical research.

Description

technical field [0001] The invention belongs to the field of airway mucus models, in particular to a method for preparing an airway mucus model. Background technique [0002] Airway mucus is a viscous colloidal liquid secreted by trachea, bronchial goblet cells and submucosal glands, which constitutes the airway surface liquid layer (Airway Surface Liquid, ASL), which covers the surface of the airway mucosa and plays a role in lubrication, moisturizing and airway protection. Airway mucus is a heterogeneous, viscoelastic colloidal liquid, more than 95% of which is water. Mucin is the main component to maintain its viscoelasticity. The molecular weight is between 300 and 32,000, which determines the rheology of airway mucus. Characteristic main substance. [0003] A preparation method of an airway mucus model is disclosed in the prior art, the patent number is CN201410328382.0, and the chemical composition and physical properties of the mucus model match the human airway mucu...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L89/00C08L1/28
CPCC08J3/246C08J2389/00C08J2489/00C08J2401/28
Inventor 徐石柯昌能
Owner 深圳市龙华区中心医院
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