The invention relates to an antistatic plastic-wood composite board and a preparation method of the antistatic plastic-wood composite board. The preparation method comprises the steps of mixing wood flour, plastic particles, maleic anhydride grafted plastic particles, glyceryl monocaprate, dodecyl diethanolamine and industrial white oil at high speed, cooling and crushing to obtain plastic-wood composite grains; and then mixing the plastic-wood composite grains, copper powder, stearic amide, talcum and zinc stearate at high speed, and carrying out fusion blending and extrusion forming by adopting an extruder. The antistatic plastic-wood composite board is convenient to produce and manufacture, low in cost, low in consumption of plastic and wood, environment-friendly, high in modulus and rigidity, creep-resistant, and excellent in antistatic effect, can be repeatedly regenerated and does not generate warping deformation. The plastic-wood composite board can be used as outdoor desks and chairs, wooden stools, picnic tables, flower tanks, guardrails, indicators and other products to be widely applied parks, courts, municipal administration and other occasions like the traditional plastic-wood boards, and can also be used in chemical industry, petroleum and other special environments.