Corn root system mutant screening method based on a splint hydroponic method

Abstract

The invention provides a corn root system mutant screening method based on a splint hydroponic method. The corn root system mutant screening method comprises the following steps: S1, disinfection of corn seeds is performed; S2, culture is performed in a splint culture device, wherein the splint culture device comprises an inner glass plate, germinating paper, an outer glass plate and fixing clamps, the germinating paper is arranged on the adjacent sides of the inner glass plate and the outer glass plate respectively, the inner glass plate and the outer glass plate are fixed through the four fixing clamps, and corn seeds are arranged between the two layers of germinating paper; S3, the characters of the corn root system are recorded: culturing of the corn seeds in a culture basin is performed for 14 days, and the related characters of the corn root system are recorded; and S4, screening and transplanting are carried out: the screened corn mutants are transplanted into a non-woven fabricculture bag. The corn mutants are cultured in a splint culture device and can be observed and recorded at any time, corn mutant materials with remarkably changed roots can be found out quickly and efficiently, damage to the corn roots is small, the survival rate is ensured and the method is convenient and practical.

Description

technical field [0001] The invention belongs to the technical field of screening methods for corn root mutants, and in particular relates to a screening method for corn root mutants based on splint hydroponics. Background technique [0002] Food security is a pressing global issue. Corn is an important food crop in the world and plays an important role in ensuring national food security. Of all the factors currently limiting maize production, the impact of climate change on water resources and diminishing supplies due to rising fertilizer costs are playing an increasingly important role. Clearly, a better understanding of the development of drought and low nutrient tolerance in maize will help identify target traits that increase yield potential while optimizing water and nutrient use efficiency. [0003] Among the morphological factors affecting tolerance to drought and low nutrients, root traits played a major role. In the review by Ludlow and Muchow (1990) some traits ...

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Problems solved by technology

However, the roll paper method also has deficiencies in the implementation process: when the roll paper wraps the corn kernels, the operation is more complicated, and it is very easy to cause the corn kernels to fall off from the top; the roll paper cannot stand upright well after absorbing water, and The fixed corn kernels are easily deformed, which affects the downward extension of the root system; secondly, when the rolling paper is unrolled, the sample is prone to slide, resulting in mixed samples

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