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A simultaneous extraction method of total flavonoids, triterpenoids and yellow pigments with high antioxidant activity

An extraction method and technology of yellow pigment, which can be applied in the direction of anti-toxic agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of inability to realize industrialization, fewer extraction and analysis processes, and inability to achieve higher extraction rates, etc. Achieve the effect of realizing high-value utilization and high antioxidant activity

Active Publication Date: 2021-12-24
JINING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, although the above prior art discloses the extraction process of yellow pigment, we have found through years of research that the final product yellow pigment obtained in the above extraction process has low antioxidant activity and free radical scavenging ability, and in many cases Can not meet medicinal standards, meanwhile, only for the extraction of yellow pigment in pomegranate peel, the technology in the prior art all can not reach higher extraction rate, can't realize industrialization
[0003] In summary, there are few studies on the extraction and analysis of active substances in plant extraction residues, and the simultaneous extraction of pomegranate peel flavonoids, triterpenes, and yellow pigments with high antioxidant activity in extraction residues by supercritical CO2 technology has not been reported yet.

Method used

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  • A simultaneous extraction method of total flavonoids, triterpenoids and yellow pigments with high antioxidant activity
  • A simultaneous extraction method of total flavonoids, triterpenoids and yellow pigments with high antioxidant activity
  • A simultaneous extraction method of total flavonoids, triterpenoids and yellow pigments with high antioxidant activity

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Embodiment 1

[0034] A method for synchronously extracting total flavonoids, triterpenes and yellow pigments with high antioxidant activity from pomegranate peel, specifically comprising the following steps:

[0035] Step 1, supercritical extraction of pomegranate peel, take the pomegranate peel powder and add it to a supercritical extractor, use 0.5mL ethanol as an entrainer, set the pressure, temperature and time conditions, collect the extract at 2mL / min, and keep the raffinate for later use;

[0036] Step 2: Ultrasonic extraction of the yellow pigment from the raffinate, put the raffinate into a conical flask, add 20% ethanol according to the ratio of 1:5 to liquid, extract it in a constant temperature water bath at 50°C for 1 hour, and pass through the ultrasonic wave with a frequency of 100KHz Treat for 20 minutes, let stand to discard the lower sediment, centrifuge the supernatant at 4000r / min for 10 minutes to obtain the ethanol extract containing yellow pigment, use a pipette to tak...

Embodiment 2

[0040] A method for synchronously extracting total flavonoids, triterpenes and yellow pigments with high antioxidant activity from pomegranate peel, specifically comprising the following steps:

[0041] Step 1, supercritical extraction of pomegranate peel, take the pomegranate peel powder and add it to a supercritical extractor, use 0.5mL ethanol as an entrainer, set the pressure, temperature and time conditions, collect the extract at 2mL / min, and keep the raffinate for later use;

[0042] Step 2: Ultrasonic extraction of the yellow pigment from the raffinate, put the raffinate into a conical flask, add 20% ethanol according to the ratio of 1:5 to liquid, extract it in a constant temperature water bath at 50°C for 1 hour, and pass through the ultrasonic wave with a frequency of 100KHz Treat for 20 minutes, let stand to discard the lower sediment, centrifuge the supernatant at 4000r / min for 10 minutes to obtain the ethanol extract containing yellow pigment, use a pipette to tak...

Embodiment 3

[0046] A method for synchronously extracting total flavonoids, triterpenes and yellow pigments with high antioxidant activity from pomegranate peel, specifically comprising the following steps:

[0047] Step 1, supercritical extraction of pomegranate peel, take the pomegranate peel powder and add it to a supercritical extractor, use 0.5mL ethanol as an entrainer, set the pressure, temperature and time conditions, collect the extract at 2mL / min, and keep the raffinate for later use;

[0048] Step 2: Ultrasonic extraction of the yellow pigment from the raffinate, put the raffinate into a conical flask, add 20% ethanol according to the ratio of 1:5 to liquid, extract it in a constant temperature water bath at 50°C for 1 hour, and pass through the ultrasonic wave with a frequency of 100KHz Treat for 20 minutes, let stand to discard the lower sediment, centrifuge the supernatant at 4000r / min for 10 minutes to obtain the ethanol extract containing yellow pigment, use a pipette to tak...

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Abstract

The invention relates to a method for synchronously extracting total flavonoids, triterpenoids and yellow pigments with high antioxidant activity. Orthogonal experiments were used to explore the effects of extraction time, pressure, temperature, and entrainer concentration on the extraction rate of total flavonoids, triterpenoids, and supercritical residue yellow pigment, and the optimal process conditions were obtained. The optimum conditions for supercritical extraction of total flavonoids were 70min, 30MPa, 85°C, entrainer concentration 90%, and the extraction rate of total flavonoids was 12.482%. The extraction rate of ultrasonic yellow pigment in the raffinate was 5.870%. The optimal condition of yellow pigment is 70min, 35MPa, 85℃, ethanol concentration 90%, the extraction rate of yellow pigment is 6.193%, and the extraction rate of total flavonoids is 12.351%. The invention provides test data and theoretical support for production, and achieves high-value utilization of waste materials such as pomegranate peels and supercritical extraction residues.

Description

technical field [0001] The invention belongs to the technical field of natural pigment extraction, and in particular relates to a method for supercritical extraction of total flavonoids and triterpenoids from pomegranate peel and a method for extracting yellow pigment with high antioxidant activity in raffinate. Background technique [0002] Pomegranate peel is rich in active substances such as flavonoids, polyphenols, and terpenoids. It has various functions such as anti-oxidation, anti-influenza virus, anti-bacteria, anti-cancer, protection of cardiovascular and cerebrovascular, and regulation of endocrine, and has great medicinal value. Pomegranate peels account for about 1 / 5 of the weight of pomegranates. Studies have shown that the main chemical components in pomegranate peels are flavonoids. The extraction of plant flavonoids comes from roots, stems, leaves, fruits, and pericarp. Common methods for the extraction and purification of flavonoids from pomegranate peel inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P39/06
CPCA61K36/185A61P39/06A61K2236/37A61K2236/55
Inventor 尹春光宋文路李明丽王晓强刘亚文
Owner JINING UNIV