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A kind of fundus vascular disease detection biomarker, detection kit and application

A technology for vascular diseases and fundus, applied in the field of biomedical detection, to achieve the effect of auxiliary diagnosis and prognosis evaluation

Active Publication Date: 2021-04-23
蒋沁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few studies on circRNA as a diagnostic marker for fundus vascular diseases

Method used

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  • A kind of fundus vascular disease detection biomarker, detection kit and application
  • A kind of fundus vascular disease detection biomarker, detection kit and application
  • A kind of fundus vascular disease detection biomarker, detection kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Application of hsa_circ_0000615 and hsa_circ_0000284 in the diagnosis of fundus vascular diseases

[0038] Aqueous humor samples and processing: Aqueous humor from 200 patients with diabetic retinopathy (PDR), aqueous humor from 200 patients with age-related macular degeneration (AMD) accompanied by choroidal neovascularization (CNV), and 200 cases of cataract ( Aqueous humor of patients without fundus lesions) was used as a control.

[0039] experiment procedure

[0040] Step 1. Obtain a sample of aqueous humor to be tested

[0041] The samples were aqueous humor samples from patients with fundus vascular diseases and cataracts.

[0042] Step 2. Extract RNA from the aqueous humor sample to be tested

[0043] a) Transfer 0.03ml aqueous humor sample to a centrifuge tube, add 1ml Trizol, pipette up and down repeatedly until the cells are completely lysed.

[0044] b) Add chloroform (1 / 5 volume of the total volume of the sample solution + Trizol Reagent) to c...

Embodiment 2

[0068] Example 2 Feasible application of hsa_circ_0000615 and hsa_circ_0000284 as prognostic markers

[0069] Step 1. Obtain a sample of aqueous humor to be tested

[0070] Aqueous humor samples from 200 patients with diabetic retinopathy (PDR) before and after anti-neovascular treatment and 200 patients with age-related macular degeneration (AMD) complicated with choroidal neovascularization (CNV) were collected.

[0071] Step 2. Extract RNA from the aqueous humor sample to be tested

[0072] a) Transfer 0.03ml aqueous humor sample to a centrifuge tube, add 1ml Trizol, pipette up and down repeatedly until the cells are completely lysed.

[0073] b) Add chloroform (sample solution + 1 / 5 volume of Trizol Reagent volume) to cover the centrifuge tube tightly, vibrate vigorously for 15 sec, and let stand at room temperature for 5 min;

[0074] c) Centrifuge at 4°C, 12,000g×15min. Carefully take out the centrifuge tube from the centrifuge. At this time, the homogenate is divided ...

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Abstract

The invention belongs to the technical field of biomedical detection, and in particular relates to a biomarker for detecting fundus vascular diseases, a detection kit and an application thereof. The kit mainly includes RNA extraction system, reverse transcription system and PCR reaction system. The kit of the present invention uses fluorescent quantitative PCR technology to detect the expression level and expression difference of two circular RNAs, namely hsa_circ_0000615 and hsa_circ_0000284, and provides new detection markers and prognosis evaluation methods for fundus vascular diseases. The kit provided has the characteristics of less trauma and simple operation.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a biomarker for detecting fundus vascular diseases, a detection kit and an application thereof. Background technique [0002] Fundus vascular disease is a kind of disease characterized by fundus vascular disease. The incidence rate is increasing year by year. It has become a global public health problem and a social and economic burden. Fundus vascular diseases include proliferative diabetic retinopathy (PDR), wet age-related macular degeneration (AMD), retinopathy of prematurity (ROP), etc. Such diseases have become the leading causes of blindness and visual impairment in all age groups from infants to the elderly, seriously affecting the quality of life of patients. The important pathological features of this type of disease are: retinal non-perfusion, increased vascular permeability and retinal neovascularization. The course of the disease usually inv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/113
CPCC12Q1/6851C12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/178C12Q2531/113C12Q2561/113C12Q2563/107C12Q2545/101
Inventor 颜标蒋沁刘明
Owner 蒋沁