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The application and products of the ratio of unc45a to hsp10 in the early warning, diagnosis and prognosis evaluation of pop

An early warning and ratio technology, applied in the fields of biotechnology and medicine, can solve problems such as the application of UNC45A/HSP10 that have not been reported in research, and achieve the effect of high accuracy, simple operation method and high specificity

Active Publication Date: 2021-06-22
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] UNC45A / HSP10 is a protein related to Duchenne muscular dystrophy (DMD). , 53-54, revealed that the treatment of DMD patients can be achieved by increasing the expression of endogenous UNC45A / HSP10, but there is no research report on the application of UNC45A / HSP10 in POP and its related products

Method used

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  • The application and products of the ratio of unc45a to hsp10 in the early warning, diagnosis and prognosis evaluation of pop
  • The application and products of the ratio of unc45a to hsp10 in the early warning, diagnosis and prognosis evaluation of pop
  • The application and products of the ratio of unc45a to hsp10 in the early warning, diagnosis and prognosis evaluation of pop

Examples

Experimental program
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Effect test

Embodiment 1

[0023] This embodiment uses the ratio of UNC45A to HSP10 (UNC45A / HSP10) as the detection index of the pelvic floor organ prolapse detection kit. Mainly, the substance for detecting the levels of UNC45A and HSP10 is used to prepare a detection kit for pelvic floor organ prolapse, so as to assist in the diagnosis, early warning and / or prognosis evaluation of POP.

[0024] Concrete preparation process steps are as follows:

[0025] (1) Prepare a multi-well plate coated with mouse anti-human UNC45A monoclonal antibody (purchased from Santa Cruz Company, Cat. No. SC-376313).

[0026] Dilute the mouse anti-human UNC45A monoclonal antibody to a concentration of 10 μg / mL with carbonate buffer solution of pH = 9, add anhydrous methanol to the antibody diluent, and add anhydrous methanol at a concentration of 3 mL / 100 mL, then add methanol-containing mouse anti-human UNC45A monoclonal antibody dilution at 100 μL / well into each well of a 96-well plate, and coat at 4°C for 12 hours; when...

Embodiment 2

[0037] In this example, the pelvic floor organ prolapse detection kit prepared in Example 1 is used to detect the samples to be tested.

[0038] 1. Detection of the concentration of UNC45A and HSP10 in the sample

[0039] (1) Standard sample preparation

[0040] The UNC45A protein standard that is equipped in the kit described in Example 1 is set as the standard sample containing the highest concentration of UNC45A, named as standard sample 1, and the concentration of UNC45A is 40ng / mL, and then used in the kit described in Example 1 Prepared sample diluent to dilute the UNC45A protein standard into UNC45A concentrations of 40 ng / mL, 20 ng / mL, 10 ng / mL, 5 ng / mL, 2.5 ng / mL, 1.25 ng / mL, 0.63 ng / mL The standard samples in mL are named standard sample 1, standard sample 2, standard sample 3, standard sample 4, standard sample 5, standard sample 6 and standard sample 7 in sequence, and there are 7 standard samples in total.

[0041] The HSP10 protein standard that is equipped in ...

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Abstract

The invention discloses the application and product of the ratio of UNC45A to HSP10 in POP early warning, diagnosis, and prognosis assessment. For the first time, the substance for detecting the ratio of UNC45A to HSP10 is used to prepare POP early warning, diagnosis, and prognosis assessment products. The operation method is simple , with blood as the detection sample, it can realize rapid and batch detection. The expression of the ratio of UNC45A to HSP10 can be realized by existing methods such as immunofluorescence analysis, ELISA, biochip method, electrochemiluminescence method, etc., with high sensitivity and high specificity High precision, high accuracy and high precision.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, specifically, the application and product of the ratio of UNC45A to HSP10 in POP early warning, diagnosis and prognosis evaluation. Background technique [0002] Female pelvic floor organ prolapse (Pelvic Organ Prolapse, POP) is the most common and most harmful type of female pelvic floor dysfunction (PFD), often accompanied by structural and functional defects of pelvic support tissue, The global incidence rate is as high as 20% and is increasing year by year. Early detection and early treatment of POP can improve the quality of life and physical and mental health of adult women. It has important guiding significance for improving the effect of POP epidemiological prevention and control, and is one of the important measures to implement the goals of the Healthy China 2030 plan. [0003] At present, the diagnosis of POP is mainly based on clinical phenotype, medical history and related...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/36G01N2800/52
Inventor 牛晓宇王涛高林波梅玲崔陶陈悦悦魏冬梅张月婷罗晓雷
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN