Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dendrobium nobile alcohol extract for regulating CD36, FABP4, SRA and PPAR gamma and application of dendrobium nobile alcohol extract

A technology of FABP4 and CD36, applied in medical preparations containing active ingredients, drug combinations, plant raw materials, etc., can solve the problem that the expression level of Dendrobium or Dendrobium extract has not been found yet

Inactive Publication Date: 2020-11-03
安徽同济生生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] According to our literature research, it has not been found that Dendrobium or Dendrobium extracts are used to regulate the expression levels of the above genes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dendrobium nobile alcohol extract for regulating CD36, FABP4, SRA and PPAR gamma and application of dendrobium nobile alcohol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare dendrobium alcohol extract, specifically as follows:

[0024] Preparation of Alcohol Extract of Dendrobium: Weigh 20g of Dendrobium Huoshanense dry powder, extract with 400mL of 75% ethanol at 55-60°C, extract twice, extract 24h each time, combine the extracts twice, and extract the combined extracts Concentrate under reduced pressure to 1 / 6-1 / 8 of the original volume, and freeze-dry to constant weight to obtain a dendrobium alcohol extract with a mass volume concentration of 100 mg / mL.

[0025] The dendrobium alcohol extract prepared in the above Example 1 with a mass concentration of 100 mg / mL was sterilized by filtration with a 0.22 μm filter membrane before the test, and then diluted to prepare a mass concentration of 10 μg / mL, 100 μg / mL, A series of 500 μg / mL dendrobium alcohol extract liquids were stored in cold storage for experiments and for future use.

[0026] (I) Detection of Dendrobium alcohol extract on the mRNA expression of CD36, FABP4, PPARγ and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of pharmacy, and relates to a dendrobium nobile alcohol extract for regulating CD36, FABP4, SRA and PPAR gamma and an application of the dendrobium nobilealcohol extract. Experimental results prove that the dendrobium nobile alcohol extract prepared by the invention can obviously inhibit the gene expression levels of a fatty acid transport protein, a fatty acid binding protein, a peroxisome proliferator-activated receptor gamma and a class A scavenger receptor, so that the dendrobium nobile alcohol extract prepared by the invention has the effect of effectively regulating the metabolism of cholesterol in the liver, and a certain theoretical basis is provided for the application of the dendrobium nobile alcohol extract to the preparation of clinical medicines for regulating the metabolism of cholesterol.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a dendrobium alcohol extract for regulating CD36, FABP4, SRA and PPARγ and its use. Background technique [0002] Fatty acid-binding protein (fatty acid-binding protein, FABP4) is a family of polygenic low molecular weight intracellular proteins, widely distributed in mammalian small intestine, liver, fat, heart, brain and skeletal muscle and other cells, with tissue specificity. FABP4 is expressed in macrophages, which have the ability to endocytose lipids and release oxides and immune mediators. Studies in FABP4 gene-deficient mice have shown that this lipid carrier is closely related to the occurrence of diseases such as metabolic syndrome, inflammation and atherosclerosis. [0003] CD36 (fatty acid transporter) is a fatty acid receptor responsible for the recognition and transmembrane transport of fatty acids. CD36 can help macrophages and tumor cells take up fat molecules ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/8984A61P3/06A61P3/00
CPCA61K36/8984A61K2236/333A61P3/00A61P3/06
Inventor 余茂耘韩际宏段亚君梁英权杨潇潇陈元利张丰陈贵兰
Owner 安徽同济生生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com