Preparation method and application of nano eye medicine

A drug and ophthalmic technology, applied in the fields of nanomedicine and biomedicine, can solve the problems of no effective effect, decreased curative effect, retinal fibrosis, etc., and achieve the effect of delaying further deterioration and improving the quality of life

Active Publication Date: 2020-11-03
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term anti-VEGF treatment not only reduces the curative effect, but also induces various complications such as retinal fibrosis and scarring.
Moreover, this method only targets the new blood vessels that appear in the late stage of wet AMD, and has no effective effect on the occurrence and development of early disease and dry AMD.

Method used

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  • Preparation method and application of nano eye medicine
  • Preparation method and application of nano eye medicine
  • Preparation method and application of nano eye medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A nanometer ophthalmic drug for pre-intervention of oxidative stress-damaged fundus diseases, the preparation method of which comprises the following steps:

[0027] Mix graphite powder and polypeptide molecules in a mass ratio of 1:2-3:1 and place them in a ball mill jar. Ball mill at room temperature for 2-4 hours at a speed of 300-500 rpm. After ball milling, high-speed centrifugation and deionized water dialysis are used respectively to remove impurities in the precipitate in turn. Finally, the precipitate is dispersed in deionized water to obtain a nitrogen-containing polypeptide-modified graphene nanosystem. from figure 1 As can be seen in the schematic diagram of , the synthesized new nanomedicine enters the cell through the size effect of atomically thick nanosheets, attracts ROS through the large π bond on the surface, and then catalyzes the elimination of ROS through the excellent activity obtained by nitrogen doping.

Embodiment 2

[0029] The nanometer product aqueous phase that embodiment 1 obtains is dispersed in deionized water, as figure 2 As shown in (a), a stable dispersion can be obtained. The dispersion liquid was stored at 4°C for more than 2 weeks, and no change was found in the dispersion liquid, indicating that the nanomedicine has good long-term aqueous phase dispersibility. After co-cultivating the prepared nano-drugs with ARPE-19 cells, a cell line of retinal pigment epithelial (RPE) cells for a certain period of time, it can be found that even after 72 hours of co-cultivation with up to 10 µg / mL of nano-drugs, the cells The survival rate is still as high as 100% ( figure 2 b), which fully demonstrates the good biocompatibility of the prepared nanomedicine.

Embodiment 3

[0031] The aqueous nano-drug dispersion obtained in Example 2 was spot-coated on a mica sheet, and then its morphology was detected by AFM. from image 3 It can be seen that the prepared nitrogen-doped polypeptide-modified graphene exhibits a typical graphene nanosheet structure with a thickness of about 1.4 nm, indicating that its excellent size effect can penetrate the cell membrane and enter the cell.

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Abstract

The invention relates to a preparation method and application of a nano eye medicine. By construction of a novel nitrogen-doped polypeptide modified graphene nano medicine with excellent catalytic activity, excessive active oxygen in retinal cells is eliminated, the oxidative stress state of the cells is relieved, and related fundus lesions are intervened in the early stage. Nitrogen-containing polypeptide molecules and graphite powder are blended, ball-milled and modified to prepare a nitrogen-doped polypeptide modified graphene nano system. Cell experiments and animal experiments prove thatthe prepared nano system has excellent scavenging capacity on excessive active oxygen in a cell model and an animal model with blue light injury, and reveals feasibility of intervening disease coursedevelopment of the novel nano system in the early stage of fundus lesion. A new thought is provided for treatment of oxidative stress injury fundus diseases.

Description

technical field [0001] The invention relates to the technical fields of nano-medicine and biomedicine, in particular to a preparation method and application of nano-medicine for ophthalmology. Background technique [0002] Oxygen is an indispensable substance in the process of human life movement, but the oxygen in the environment has a high oxidative property, which will cause various organs of the human body to be continuously corroded by oxygen and aging damage with age. The main cause of this damage is the excessive production of reactive oxygen species (ROS) in the body. ROS is a kind of one-electron reduction product of oxygen in the body, which is generated by electrons leaking out of the respiratory chain and consuming a small amount of oxygen before being delivered to the terminal oxidase. ROS radicals are a class of molecules containing lone pairs of electrons in outer orbitals, including superoxide anion (O 2- ), hydrogen peroxide (H 2 o 2 ), hydroxyl radical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/64A61K47/69A61K33/44A61P27/02A61P27/06A61P39/06A61P3/10
CPCA61K47/64A61K47/6953A61K33/44A61P27/02A61P27/06A61P39/06A61P3/10
Inventor 刘勇单素艳晏露
Owner WENZHOU MEDICAL UNIV
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