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Tuberculosis markers and their application

A tuberculosis and marker technology, applied in the field of biotechnology detection, can solve problems such as difficult to distinguish

Active Publication Date: 2022-02-01
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as mentioned above, tuberculosis, lung adenocarcinoma, and pulmonary sarcoidosis are not easy to distinguish in many cases, so effective disease-specific lung tissue biomarkers are needed for auxiliary detection and judgment

Method used

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  • Tuberculosis markers and their application
  • Tuberculosis markers and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 MMP8 protein is used to identify diseased lung tissue of tuberculosis patients

[0068] 1) Obtain lung tissue samples (n=10, 12 and 8) from patients with pulmonary tuberculosis (TB), lung adenocarcinoma (AD) and pulmonary sarcoidosis (SA) to be tested, and use the samples of lung adenocarcinoma patients Adjacent normal tissue was used as normal lung tissue control (NC; n = 8).

[0069] 2) The lung tissue samples were fixed with formalin, and then the tissue samples were embedded in paraffin.

[0070] 3) Carry out serial sections of paraffin, one part is subjected to hematoxylin-eosin (H&E) staining for pathological analysis, and the other part is subjected to immunohistochemical method determination, and the antibody that specifically recognizes MMP8 protein can be selected from the anti-MMP8 antibody from Santa Cruz Company ,Specific steps are as follows

[0071] (a) Paraffin sections were dewaxed to water: put the sections in xylene I for 15 minutes, xylen...

Embodiment 2

[0084] Preparation and use of embodiment 2 pulmonary tuberculosis detection kit

[0085] The existing anti-MMP8 protein antibody is used, or the monoclonal antibody or polyclonal antibody specifically binding to the MMP8 protein is prepared by conventional techniques.

[0086] The kit contains: detectable signal molecules and detection antibodies that can bind to detectable signal molecules, monoclonal or polyclonal antibodies used to recognize and specifically detect MMP8 protein, reactants that react with detection antibodies and signal molecules, and antigen retrieval solution , buffer solution, etc.; the detectable molecule can be selected from general chemical substances in this field, for example; horseradish peroxidase, alkaline phosphatase, glucose oxidase, β-D-galactosidase, urease, acridine Esters, luminol; choose suitable reactants according to the characteristics of different detectable molecules, such as o-phenylenediamine (OPD), tetramethylbenzidine (TMB), lumino...

Embodiment 3

[0089] Example 3 BRCA1 and PCNA proteins are used in the diagnosis of lung adenocarcinoma lesions

[0090] 1) Obtain lung tissue samples (n=10, 12 and 8) from patients with pulmonary tuberculosis (TB), lung adenocarcinoma (AD) and pulmonary sarcoidosis (SA) to be tested, and use the samples of lung adenocarcinoma patients Adjacent normal tissue was used as normal lung tissue control (NC; n = 8).

[0091] 2) The lung tissue samples were fixed with formalin, and then the tissue samples were embedded in paraffin.

[0092] 3) The paraffin was serially sectioned, one part was subjected to H&E staining pathological analysis according to the standard method, and the other part was subjected to immunohistochemical method to detect the expression levels of BRCA1 and PCNA according to the standard method. The immunohistochemical detection method is the same as in Example 1, except that the MMP8 antibody is replaced by an antibody that specifically recognizes BRCA1 protein or PCNA prote...

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Abstract

The invention discloses a group of tuberculosis and lung adenocarcinoma markers and their applications, belonging to the technical field of biotechnology detection. The present invention finds that the marker of pulmonary tuberculosis is the highly expressed MMP8 protein in lung tissue; the lung adenocarcinoma marker is a combination of one or both of the highly expressed BRCA1 and PCNA proteins in lung tissue; the marker of the present invention can assist The use of biopsy to help diagnose related lung diseases has good clinical application prospects, especially in the development of products such as kits for the diagnosis and identification of the lung diseases.

Description

technical field [0001] The invention relates to markers of tuberculosis and lung adenocarcinoma and their applications, in particular to the development of products such as kits for diagnosing tuberculosis, lung adenocarcinoma and pulmonary sarcoidosis using patient lung tissue samples, and belongs to the technical field of biotechnology detection. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Currently, about a quarter of the world's population is infected with tuberculosis, and more than one million people die from tuberculosis every year. Among tuberculosis patients, about 85% belong to tuberculosis infection. Clinically, pulmonary tuberculosis has very similar symptoms, pathological features, and imaging findings to many other chronic lung diseases, such as lung cancer and pulmonary sarcoidosis, which brings great challenges to differential diagnosis. Lung cancer is the type of cancer that causes the most huma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574C12Q1/6886C12Q1/6883
CPCG01N33/6893G01N33/57484G01N33/57488G01N33/57423C12Q1/6886C12Q1/6883C12Q2600/158C12Q2600/136G01N2800/12
Inventor 刘翠华柴琪瑶陆喆汪静
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI