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A kind of injectable rare earth-DNA photothermal gel, its preparation method and application

A technology for injection and DNA molecules, which is applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas. The complex fluid environment and other problems can achieve the effect of high-efficiency photothermal therapy, low probability of tumor recurrence and metastasis, and simple and fast operation

Active Publication Date: 2021-09-21
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional treatment mode of tail vein injection, the cumulative concentration of nanomaterials at the tumor site is low, which greatly weakens the effect of photothermal therapy on the tumor site
In addition, the circulation of photothermal materials in the body will have certain toxic and side effects on normal tissues and organs.
In particular, some nanomaterials pose long-term risks to organisms due to their non-degradability
In addition, due to the complex fluid environment in the urothelial bladder, it is difficult to treat UBC with photothermal agents

Method used

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  • A kind of injectable rare earth-DNA photothermal gel, its preparation method and application
  • A kind of injectable rare earth-DNA photothermal gel, its preparation method and application
  • A kind of injectable rare earth-DNA photothermal gel, its preparation method and application

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preparation example Construction

[0046] The present invention does not limit the preparation method of the UCNP nanoparticles, and methods well-known to those skilled in the art will suffice.

[0047] Preferably specifically: YCl 3 , YbCl 3 , ErCl 3 , oleic acid and octadecene were mixed, stirred, heated to 140-160°C under nitrogen protection, maintained for 30-40 minutes, and then lowered to 40-50°C to obtain the first mixed solution;

[0048] Take NH 4 F and NaOH were dissolved in methanol, after ultrasonic mixing, mixed with the first mixed solution, stirred at 50°C for 1-2 hours, then evaporated to remove the solvent, washed to obtain solution A;

[0049] YCl 3 , NdCl 3 Mix oleic acid and octadecene, stir, raise the temperature to 160°C under nitrogen protection, maintain it for 30-40min, and then lower it to 40-50°C to obtain the second mixed solution;

[0050] Take NH 4 Dissolve F and NaOH in methanol, ultrasonically mix, heat and stir with the second mixed liquid and solution A, heat up to evapo...

Embodiment 1

[0091] The present invention provides a hydrogel with good biocompatibility, injectability, remarkable photothermal conversion effect and photodynamic effect, which is composed of negatively charged DNA molecules—salmon sperm DNA and UCNP with a large number of positive charges - Made of Au nanoparticles, the specific preparation process is as follows:

[0092] 1. Synthesis of UCNP-Au nanoparticles with a large number of positive charges:

[0093] The first step: prepare the core-shell type hexagonal phase-rare earth doped fluorescent upconversion nanomaterial (βOA-UCNP) by high temperature oil solvent method. The details are as follows: Take 152.3mg of YCl3, 55.88mg of YbCl3 and 5.47mg of ErCl3 into a 100mL round bottom flask, then add 6ml of oleic acid and 18ml of octadecene, and keep stirring. After exhausting the gas, the temperature was raised to 160°C for 40 minutes under the protection of nitrogen, and then lowered to 50°C. Take 149mg NH 4 F and 100mg NaOH were disso...

Embodiment 2

[0103] Example 2 is similar to Example 1, except that the mass fraction ratio of salmon sperm DNA (2000bp) to UCNP-Au nanoparticles is 15:1.

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Abstract

The invention provides an injectable rare earth-DNA photothermal gel, comprising: negatively charged DNA molecules and positively charged rare earth-based nanomaterials; the positively charged rare earth-based nanomaterials are UCNP-Au . Under the action of physical entanglement, hydrogen bond and electrostatic force, DNA molecules and rare earth-based nanomaterials in the present invention form a hydrogel material with injectability, high-efficiency photothermal treatment effect and biocompatibility. Experimental results show that the photothermal efficiency of the injectable rare earth-DNA photothermal gel in the present invention can reach 42.67%. In addition, the tumor suppression rate is increased, thereby reducing the chance of tumor recurrence and metastasis. By means of paratumoral injection, the toxic and side effects of drugs on normal tissues during treatment can be reduced. Moreover, by adjusting the ratio of DNA molecules and rare earth-based nanomaterials, the degradation of photothermal gels in any time period can be realized. The operation of the invention is simple and fast, and the probability of tumor recurrence and metastasis is low.

Description

technical field [0001] The invention relates to the technical field of self-assembly of natural biological macromolecules and emerging rare earth nanocomposite materials, in particular to an injectable rare earth-DNA photothermal gel, its preparation method and application. Background technique [0002] Urothelial bladder carcinoma (UBC) is the most common malignant tumor in the urinary system, among which non-muscle-invasive type is the most common. For this type of bladder cancer, transurethral resection of bladder tumor (TURBT) has become the most important treatment because of its simplicity and ease of operation. However, due to incomplete resection, there is a high rate of tumor recurrence after surgery, and regular intravesical infusion of chemotherapy drugs is often required. In addition, the frequent application of chemotherapy drugs will not only cause great side effects on the human body, but also lead to the increase of drug resistance, which is extremely unfavo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K47/26A61K41/00A61P35/00
CPCA61K9/0019A61K9/06A61K41/0052A61K47/26A61P35/00
Inventor 刘凯刘斌王帆张洪杰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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