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Calcium Carbonate-Based Composite Microparticles and Its Preparation and Application

A technology of calcium carbonate glycolate and microparticles, which is applied in the direction of drug combination, active ingredients of aluminum/calcium/magnesium, wave energy or particle radiation treatment materials, etc., can solve problems such as toxic and side effects, and achieve uniform particle size, good stability, The effect of increasing content

Active Publication Date: 2021-07-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radiotherapy and chemotherapy have obvious toxic and side effects, and can easily lead to drug resistance in tumors. Therefore, the development of a safe and efficient radiofrequency ablation combined treatment strategy is of great importance for further broadening the scope of application of existing radiofrequency ablation techniques in the clinical treatment of liver cancer. the meaning of

Method used

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  • Calcium Carbonate-Based Composite Microparticles and Its Preparation and Application
  • Calcium Carbonate-Based Composite Microparticles and Its Preparation and Application
  • Calcium Carbonate-Based Composite Microparticles and Its Preparation and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment one: the preparation of polylactic acid-glycolic acid calcium carbonate particle

[0067] After weighing PLGA and PLGA-PEG according to the mass ratio of 1:1, dissolve them in dichloromethane to prepare a 30mg / ml mixed solution of PLGA and PLGA-PEG. Weigh NaHCO 3 with CaCl 2 Separately dissolved in water to prepare 0.625M NaHCO 3 Aqueous solution with 1.25M CaCl 2 aqueous solution. The prepared NaHCO 3 Aqueous solution and CaCl 2 The aqueous solution was mixed with PLGA and PLGA-PEG mixed solution according to the volume ratio of 1:3, and was ultrasonically emulsified with an ultrasonic cell disruptor for 297s under the condition of ultrasonic power of 100W to obtain emulsion A (NaHCO 3 @PLGA-PEG) and Emulsion B (CaCl 2 @PLGA-PEG). Emulsion A and emulsion B were mixed and ultrasonically emulsified with an ultrasonic cell disruptor for 297s under the condition of ultrasonic power of 100W to obtain emulsion C. The obtained emulsion C was dispersed in 1...

Embodiment 2

[0068] Embodiment two: the property detection of polylactic acid-glycolic acid calcium carbonate particle

[0069] The polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 were qualitatively detected, and transmission electron microscope detection, dynamic light scattering and proton neutralization ability detection were respectively carried out.

[0070] Among them, the results of transmission electron microscope detection are as follows: figure 1 As shown in a, the results show that the polylactic acid-glycolic acid calcium carbonate microparticles obtained in Example 1 are distributed in a monodisperse state in water, showing that the particle diameter of the polylactic acid-glycolic acid calcium carbonate microparticles prepared by the present invention is uniform. Disperses well.

[0071] The results of dynamic light scattering were as follows: figure 1 Shown in b, the result shows that the polylactic acid-glycolic acid calcium carbonate particl...

Embodiment 3

[0073] Example 3: Behavioral detection of polylactic acid-glycolic acid calcium carbonate particles in mice

[0074] The behavior of the polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 in mice was detected. Firstly, the detection of blood circulation was carried out. Polylactic acid-glycolic acid calcium carbonate microparticles labeled with fluorescent dye DIR were intravenously injected into mice, and the blood of mice was collected at different time points to detect the fluorescence intensity in the blood. The result of blood circulation is as figure 2 As shown in a, the results show that the polylactic acid-glycolic acid calcium carbonate particles prepared in Example 1 have longer blood circulation time in mice after intravenous injection. Tumor enrichment results such as figure 2 As shown in b, figure 2 In b, the fluorescence at the tumor site is indicated within the dotted circle. The results of fluorescence imaging of tumor-bearin...

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Abstract

The invention relates to a calcium carbonate-based composite particle and its preparation and application. The calcium carbonate-based composite particles of the present invention include calcium carbonate and a polymer stabilization layer, wherein the polymer stabilization layer includes amphiphilic molecules and / or hydrophilic molecules. The invention also discloses the application of one or more of the calcium carbonate-based complex particles in the preparation of tumor immunotherapy adjuvant, chemotherapy enhancing adjuvant, radiotherapy enhancing adjuvant and radiofrequency ablation therapy adjuvant. The calcium carbonate-based composite particle of the present invention has a good effect of regulating the slightly acidic environment of tumors, can reduce the content of immunosuppressive cells in tumors, increase the content of immune cells capable of killing tumors, and promote the formation of tumor-associated macrophages from M2 Polarization to the M1 type can effectively reverse the tumor immunosuppressive microenvironment, activate anti-tumor immunity, and enhance the inhibitory effect of various treatments such as immunotherapy, chemotherapy, radiotherapy, and radiofrequency ablation on tumor growth.

Description

technical field [0001] The invention relates to the field of tumor treatment preparations, in particular to a calcium carbonate-based composite particle and its preparation and application. Background technique [0002] Tumor immunotherapy is a treatment that inhibits tumor growth by activating the body's own immune system. Its core is to effectively activate the anti-tumor response of T lymphocytes in tumor patients to achieve specific inhibition and killing of tumor growth. After years of development, cancer immunotherapy can be mainly divided into four aspects: immunomodulators, immune checkpoint blockade therapy, adoptive immunotherapy and immune vaccines. [0003] Although activating the body's immune function to destroy cancer cells has broad prospects, traditional immunotherapy has also exposed many toxic side effects related to excessive immunity, such as immune checkpoint inhibitors. The response is low, which is only effective for some patients efficient. The use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61K31/4188A61K31/704A61K39/39A61K41/00A61K45/00A61K47/02A61K47/34A61P35/00
CPCA61K9/167A61K9/1611A61K9/1647A61K33/10A61K39/39A61K41/00A61K45/00A61K31/704A61K31/4188A61P35/00A61K2039/55505A61K2039/55555A61K2300/00
Inventor 刘庄冯良珠杨志娟郝钰朱宇杰
Owner SUZHOU UNIV
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