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Fusion protein and application thereof

A fusion protein and application technology, applied in the direction of fusion polypeptide, peptide/protein components, animal/human protein, etc., can solve the problems of atherosclerosis that have not yet been seen, achieve the effect of reducing the plaque area and delaying the progress of the disease

Inactive Publication Date: 2020-11-10
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no relevant report on the treatment of atherosclerosis with SIRPα-Fc fusion protein

Method used

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  • Fusion protein and application thereof
  • Fusion protein and application thereof
  • Fusion protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Cell Modeling:

[0047] When RAW264.7 and MOVAS cells are in the logarithmic growth phase, after trypsinization and centrifugation, the cells are resuspended in 3% serum, seeded in a 6-well plate at a concentration of 10,000 / ml, and 25, 50, and 75 μg / ml of oxidation After 24 hours of low-density lipoprotein, oil red O staining was used to test whether the model was successfully established; the results were as follows: figure 1 As shown, after adding different concentrations of ox-LDL, the oil red O staining part in the cells increased significantly, and it was dose-dependent; the results showed that the modeling method can induce foam cells, and at 50 μg / mL, At a concentration of 75 μg / mL, the modeling rate can reach over 90%.

[0048] The expression of CD47 in successfully modeled cells was detected by Western Blot:

[0049] After successful modeling, the cells were scraped off with a cell scraper, collected and lysed, and checked by Western Blot; the results were a...

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PUM

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Abstract

The invention belongs to the field of medicines, and relates to a novel fusion protein. The fusion protein is combined with CD47 molecules on the surfaces of deposited cells in atherosclerotic plaquesto block the transmission of signals to macrophages, relieve the inhibition of a phagocytic function of macrophages, promote the macrophages to phagocytize the deposited cells in the atheroscleroticplaques, and delay and reverse the progress of the atherosclerosis course. Animal experiments of atherosclerosis early-stage and middle-late-stage model mice prove that after the fusion protein is injected, the aorta total length, the aorta root and the plaque area at the aortic arch of an experimental mouse are remarkably reduced. The fusion protein can be used for delaying and reversing the progress of the disease course of an atherosclerosis model animal and reducing related acute cardiovascular events of the atherosclerosis model animal, and further can be used for preparing a new medicinefor clinically treating atherosclerosis.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a new type of fusion protein. The fusion protein of the invention is combined with CD47 molecules deposited on the surface of cells in atherosclerotic plaques to block its transmission of signals to macrophages and relieve its effect on macrophages. Inhibition of cell phagocytosis promotes macrophages to phagocytose deposition cells in atherosclerotic plaques, delaying and reversing the progression of atherosclerosis. It is further used in the preparation of new drugs for the clinical treatment of atherosclerosis. Background technique [0002] The prior art discloses that atherosclerosis is one of the most common cardiovascular diseases, and is also the pathological basis leading to acute cardiovascular events and death. The pathological manifestation of atherosclerosis is lipid deposition in the subendothelium, causing macrophages to form foam cells due to phagocytosis of lipids, and form...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/17A61K47/68A61P9/10G01N33/68
CPCC07K14/70503A61P9/10G01N33/6896C07K2319/30A61K38/00G01N2333/70503G01N2800/323G01N2800/324
Inventor 鞠佃文梁嫣煦韩磊范佳君朱泽国
Owner FUDAN UNIV
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