Application of phenylthiazine and its salts in the preparation of antitumor drugs
An anti-tumor drug, the technology of phenylthiazine, applied in the field of biomedicine, can solve the problems of no reports
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[0024] The technical solutions of the present invention will be further described in detail below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.
[0025] application test
[0026] Materials and Methods
[0027] 1. Materials
[0028] 1.1 Cells and Reagents
[0029] Esophageal squamous cell carcinoma cells KYSE150 and KYSE450 come from the Department of Pathophysiology, School of Basic Medicine, Zhengzhou University. The present invention uses a human esophageal carcinoma tissue specimen, numbered EG20. The patient was from Henan Cancer Hospital, male, 46 years old, case stage T2N0M0II, moderately differentiated.
[0030] Pizothiazine Malate Powder American ABMOLE
[0031] Penicillin North China Pharmaceutical Co., Ltd.
[0032] Streptomycin Shandong Lukang Pharmaceutical Co., Ltd.
[0033] RPMI-1640 Medium Israel Biological Industries Company
[0034] DMEM medium Biological Industries, Israel
[0035] 0.25% tr...
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