Animal cell hollow fiber culture system and cell culture method thereof

Abstract

The invention discloses an animal cell hollow fiber culture system and a cell culture method thereof. The system comprises a hollow fiber cylinder, a liquid storage system, circulating systems (an inner circulating system and an outer circulating system) and a detection control system. The hollow fiber cylinder performs culture medium circulation through the inner circulating system; and the outercirculating system is used for cell inoculation and cell digestion, uniform distribution of cells among hollow fibers is achieved through circulation of a peristaltic pump in the outer circulating system, and the cell inoculation uniformity is improved. Conditions similar to in-vivo growth are established, and the culture effect is good. An alternate positive pressure circulating system (mode) isadopted to guarantee alternate replacement of nutrient components in hollow fiber inner and outer systems, and the problems that hollow fibers are blocked and the dead volume of a nutrient solution cannot be replaced are also avoided. Meanwhile, the system is of a closed design, is good in biological safety, can effectively control pH, dissolved oxygen and temperature in the culture process, andis suitable for animal cell culture in vaccine and antibody production.

Description

technical field [0001] The invention relates to the technical field of animal cell culture, in particular to an animal cell hollow fiber culture system suitable for vaccine and antibody production and a cell culture method thereof. Background technique [0002] Commonly used cell culture methods are divided into adherent culture and suspension culture. For suspension cell culture, the scale-up of bioreactor can be used to realize the scale-up culture of suspension cells. However, when there is no suitable suspension cell line, adherent cell culture is also a vaccine. The main mode of production and antibody production. Commonly used industrial cell attachment culture methods include spinner bottle culture and fixed bed reactor culture. Spinner bottle culture is no longer approved for use in biomedicine due to its complicated operation, large differences between batches, and risks such as easy contamination. However, fixed-bed bioreactors used for adherent cell culture need ...

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Problems solved by technology

[0003] Aiming at the problem that it is difficult to realize enlarged culture due to low cell density and poor cell distribution uniformity during cell adherent culture, the present invention designs a hollow fiber culture system for animal cell adherent culture

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