Immune cell capable of secreting bispecific antibody and application of immune cell

A bispecific antibody, immune cell technology, applied in the field of biological cells, can solve the problems of no good treatment effect, low remission rate, off-target, etc., to avoid autoimmune diseases, enhance curative effect, and enhance stability.

Inactive Publication Date: 2020-11-17
GUANGDONG XIANKANGDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although CAR-T cell therapy is brilliant in the treatment of hematological tumors, it does not have a good therapeutic effect on solid tumors, with a low response rate and prone to off-target and other toxic side effects

Method used

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  • Immune cell capable of secreting bispecific antibody and application of immune cell
  • Immune cell capable of secreting bispecific antibody and application of immune cell
  • Immune cell capable of secreting bispecific antibody and application of immune cell

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The preparation method and functional verification of immune cells in this embodiment include the following steps:

[0079] Step 1: Isolation of peripheral blood PBMCs and culture of T cells

[0080] Mononuclear cells were isolated from donor peripheral blood, density gradient centrifugation was performed using ficol, and T cells were enriched with a T cell sorting kit (CD3 MicroBeads, human-lyophilized, 130-097-043), using a conjugated anti-CD3 / anti-CD28 magnetic beads activated culture and expansion of T cells; the medium used TexMACS GMP Medium (MiltenyiBiotec, 170-076-309), containing 10% FBS, 2mM L-glutamine, 100IU / ml rhIL2, all cells were placed Cultured in a 37°C, 5% CO2 constant temperature incubator to obtain T cells.

[0081] Step 2: Cell Line Culture

[0082] Cell line expressing GPC3: Huh-7 (human liver cancer cell), purchased from ATCC.

[0083] Cell line not expressing GPC3: A549 (human non-small cell lung cancer cells), purchased from ATCC.

[0084] ...

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Abstract

The invention relates to the technical field of biological cells, in particular to an immune cell capable of secreting a bispecific antibody and application of the immune cell; the immune cell capableof secreting the bispecific antibody comprises an immune cell expression chimeric antigen receptor and expresses a secreted bispecific antibody. By secreted bispecific antibodies, two negative feedback pathways of PD-1 and CTLA-4 are blocked, so that negative regulation and control of a tumor microenvironment on immune cells are weakened, depletion of the immune cells is reduced, the tumor killing function of the immune cells is enhanced, and inhibition of the tumor microenvironment on the immune cells is reduced.

Description

technical field [0001] The invention relates to the technical field of biological cells, in particular to immune cells that can secrete antibodies and express CAR, optimize the killing function of immune cells, reduce exhaustion, and reduce the inhibition of immune cells by the tumor microenvironment. Background technique [0002] In recent years, tumor immunotherapy has developed rapidly, especially adoptive cell therapy (ACT), which means that immune cells such as T cells and NK cells are isolated from patients, modified and expanded in vitro, and then reintroduced into patients for tumor treatment. method of treatment. In 2013, the immunotherapy of tumors was rated as the first among the "Top Ten Breakthroughs" by Science Impurities. [0003] CAR-T and TCR-T are important components of adoptive cell therapy, especially CAR-T therapy, which has made remarkable achievements in the treatment of blood tumors and achieved a high remission rate. A typical CAR structure consist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N5/078C12N15/13A61K39/395A61P35/00
CPCA61K2039/505A61K2039/5154A61K2039/5156A61K2039/5158A61P35/00C07K14/7051C07K16/2818C07K16/46C12N5/0634
Inventor 马丽雅薛卫巍谢海涛
Owner GUANGDONG XIANKANGDA BIOTECH CO LTD
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