Immune cell capable of secreting bispecific antibody and application of immune cell
A bispecific antibody, immune cell technology, applied in the field of biological cells, can solve the problems of no good treatment effect, low remission rate, off-target, etc., to avoid autoimmune diseases, enhance curative effect, and enhance stability.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0078] The preparation method and functional verification of immune cells in this embodiment include the following steps:
[0079] Step 1: Isolation of peripheral blood PBMCs and culture of T cells
[0080] Mononuclear cells were isolated from donor peripheral blood, density gradient centrifugation was performed using ficol, and T cells were enriched with a T cell sorting kit (CD3 MicroBeads, human-lyophilized, 130-097-043), using a conjugated anti-CD3 / anti-CD28 magnetic beads activated culture and expansion of T cells; the medium used TexMACS GMP Medium (MiltenyiBiotec, 170-076-309), containing 10% FBS, 2mM L-glutamine, 100IU / ml rhIL2, all cells were placed Cultured in a 37°C, 5% CO2 constant temperature incubator to obtain T cells.
[0081] Step 2: Cell Line Culture
[0082] Cell line expressing GPC3: Huh-7 (human liver cancer cell), purchased from ATCC.
[0083] Cell line not expressing GPC3: A549 (human non-small cell lung cancer cells), purchased from ATCC.
[0084] ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com