Construction method of rabies virus G protein-goatpox virus recombinant vaccine

A technology of rabies virus and sheep pox virus, which is applied in the field of medicine, can solve problems such as complicated procedures, long cycle times, and difficult promotion and use in flocks

Active Publication Date: 2020-11-17
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the current rabies vaccine can effectively prevent the occurrence of rabies, the cost of injection of ...

Method used

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  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine
  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine
  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine

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Embodiment Construction

[0092] The technical solution of the present invention will be described in further detail below in conjunction with the embodiments.

[0093] A method for constructing rabies virus G protein-capeppox virus recombinant vaccine, comprising the following steps:

[0094] Step 1, recombinant virus vaccine construction model design

[0095] According to the design principle of poxvirus recombinant vaccine, the TK gene of sheep pox virus was selected as the non-essential region for foreign gene insertion, the bidirectional promoter of vaccinia virus VV7.5 and goat pox virus A8R was used as the alternative promoter, and the green fluorescent photoprotein was used as the reporter gene , construct the expression vector of recombinant virus with rabies virus G protein. In order to screen the most selected vaccine combination, a total of 10 combinations were designed, and the G protein was selected in two forms of full length and extramembrane region. Specific design models such as fi...

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Abstract

The invention discloses a construction method of a rabies virus G protein-goatpox virus recombinant vaccine. The construction method comprises the following steps of: 1, design of a recombinant virusvaccine construction model; 2, amplification of each gene and fusion amplification of each combined gene; 3, construction of various combined recombinant transfer vectors; 4, recombination of rabies virus G protein and goatpox virus and virus purification; 5, rabies virus G protein expression detection; and step 6, detection of the immune effect of a rabies virus G protein-goatpox recombinant virus vaccine strain. The vaccine constructed by the method can effectively prevent rabies of goat, has the characteristics of high production efficiency, simplicity, convenience and practicability, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for constructing a rabies virus G protein-oceppox virus recombinant vaccine. Background technique [0002] Rabies is a highly lethal and contact zoonotic disease caused by the rabies virus that infects almost all mammals. my country is one of the countries with a high incidence of rabies. In addition, the territory is rich and vast, and wild animals such as wolves and foxes are more likely to carry rabies virus. Therefore, cattle and sheep in pastoral areas are attacked by wild animals and infected with rabies. In addition, the pet industry is booming. There are also reports of pet dogs biting sheep and those who develop the disease. For example, in Shanxi, Inner Mongolia, Xinjiang and other places, there are cases of cattle and sheep being bitten by dogs and infected with rabies. Goatpox virus is a member of the Poxviridae family and is a large linear DNA virus. Its gen...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/47C12N15/65C12N5/071A61K39/295A61K39/205A61K39/275A61P31/20A61P31/14
CPCC12N7/00C07K14/005C12N15/65C12N5/0683A61K39/12A61P31/20A61P31/14A61K2039/552A61K2039/5254A61K2039/70C12N2760/20122C12N2760/20134C12N2710/24221C12N2710/24234C12N2830/205
Inventor 李有文童剑军何川川张雪萍米丽开姆·托合提尼亚孜高娜杨勇飞
Owner TARIM UNIV
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