Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of rabies virus G protein-goatpox virus recombinant vaccine

A technology of rabies virus and sheep pox virus, which is applied in the field of medicine, can solve problems such as complicated procedures, long cycle times, and difficult promotion and use in flocks

Active Publication Date: 2020-11-17
TARIM UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the current rabies vaccine can effectively prevent the occurrence of rabies, the cost of injection of rabies vaccine is high, the cycle is long, and the procedure is complicated, so it is difficult to promote the use in flocks

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine
  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine
  • Construction method of rabies virus G protein-goatpox virus recombinant vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0092] The technical solution of the present invention will be described in further detail below in conjunction with the embodiments.

[0093] A method for constructing rabies virus G protein-capeppox virus recombinant vaccine, comprising the following steps:

[0094] Step 1, recombinant virus vaccine construction model design

[0095] According to the design principle of poxvirus recombinant vaccine, the TK gene of sheep pox virus was selected as the non-essential region for foreign gene insertion, the bidirectional promoter of vaccinia virus VV7.5 and goat pox virus A8R was used as the alternative promoter, and the green fluorescent photoprotein was used as the reporter gene , construct the expression vector of recombinant virus with rabies virus G protein. In order to screen the most selected vaccine combination, a total of 10 combinations were designed, and the G protein was selected in two forms of full length and extramembrane region. Specific design models such as fi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a construction method of a rabies virus G protein-goatpox virus recombinant vaccine. The construction method comprises the following steps of: 1, design of a recombinant virusvaccine construction model; 2, amplification of each gene and fusion amplification of each combined gene; 3, construction of various combined recombinant transfer vectors; 4, recombination of rabies virus G protein and goatpox virus and virus purification; 5, rabies virus G protein expression detection; and step 6, detection of the immune effect of a rabies virus G protein-goatpox recombinant virus vaccine strain. The vaccine constructed by the method can effectively prevent rabies of goat, has the characteristics of high production efficiency, simplicity, convenience and practicability, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for constructing a rabies virus G protein-oceppox virus recombinant vaccine. Background technique [0002] Rabies is a highly lethal and contact zoonotic disease caused by the rabies virus that infects almost all mammals. my country is one of the countries with a high incidence of rabies. In addition, the territory is rich and vast, and wild animals such as wolves and foxes are more likely to carry rabies virus. Therefore, cattle and sheep in pastoral areas are attacked by wild animals and infected with rabies. In addition, the pet industry is booming. There are also reports of pet dogs biting sheep and those who develop the disease. For example, in Shanxi, Inner Mongolia, Xinjiang and other places, there are cases of cattle and sheep being bitten by dogs and infected with rabies. Goatpox virus is a member of the Poxviridae family and is a large linear DNA virus. Its gen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/01C12N15/47C12N15/65C12N5/071A61K39/295A61K39/205A61K39/275A61P31/20A61P31/14
CPCC12N7/00C07K14/005C12N15/65C12N5/0683A61K39/12A61P31/20A61P31/14A61K2039/552A61K2039/5254A61K2039/70C12N2760/20122C12N2760/20134C12N2710/24221C12N2710/24234C12N2830/205
Inventor 李有文童剑军何川川张雪萍米丽开姆·托合提尼亚孜高娜杨勇飞
Owner TARIM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com