High-efficiency and high-speed analysis type counter-current chromatograph

Abstract

The invention discloses a high-efficiency and high-speed analysis type counter-current chromatograph. A metal unwinding frame and a dynamic balance adjusting plate are arranged on an existing chromatograph; the metal unwinding frame comprises a metal base, a metal straight pipe, a center shaft oil plug, two symmetrically-arranged metal bent pipes and two separation column oil plugs, one end of each metal bent pipe is fixedly connected with the metal base, one end of each separation column oil plug is connected with a center hole of the corresponding separation column in an expanding mode, andthe other end of each separation column oil plug is arranged in the other end of the corresponding metal bent pipe. One end of the metal straight pipe is fixedly connected with the metal base, one endof the central shaft oil plug is in expansion connection with one end of the central shaft, the other end of the central shaft oil plug is arranged in the other end of the metal straight pipe, the metal straight pipe is communicated with the two metal bent pipes, and lining hoses filled with grease are arranged in the metal straight pipe and the two metal bent pipes; and the metal base is connected with the planet carrier through a dynamic balance adjusting plate. The chromatograph can realize counter-current chromatography separation at an ultra-high rotating speed, and has the characteristics of small size and high reliability.

Description

technical field [0001] The invention relates to the technical field of an analytical countercurrent chromatograph, in particular to an efficient and high-speed analytical countercurrent chromatograph. Background technique [0002] Countercurrent chromatography technology is a continuous and efficient liquid-liquid partition chromatography separation technology developed in the 1970s. It generates a two-dimensional force field through synchronous planetary motion of simultaneous revolution and rotation, and retains one of the two phases. As a stationary phase, separation and extraction are achieved during high-speed rotation. [0003] Since the countercurrent chromatography technology needs to generate a two-dimensional force field that revolves and rotates simultaneously, the countercurrent chromatographs in the prior art often use a planetary gear mechanism as the main structure (such as the structure disclosed in PCT patent WO03 / 086639A2). The structure includes a support...

AI Technical Summary

Problems solved by technology

However, due to the unwinding of the flyleads unwinding tube used in the prior art countercurrent chromatograph, it is prone to fatigue damage at a slightly high speed (over 2500r / min), which in turn leads to the interruption of the entire countercurrent chromatographic process, and the separation cannot be achieved. However, the countercurrent chromatograph of the prior art cannot achieve ultra-high speed separation

At the same time, the countercurrent chromatograph in the prior art is limited by the structure of the flyleads unwinding tube, so it can only be set up with a cantilever structure; especially at ultra-high speeds, the position of the center of gravity of the flyleads unwinding tube will vary with the rotation process. Changes make it difficult to guarantee the overall dynamic balance, poor stability and low reliability during equipment operation

In addition, in some experimental environments, in addition to requiring the chromatograph to meet the ultra-high-speed separation capability, there are also certain requirements for the amount of sample separation. However, the countercurrent chromatograph in the prior art can only use A single separation column (if two separation columns are used at the same time, the flyleads unwinding tubes from the two separation columns will interfere with each other under high-speed operation), to meet the requirements of experimental analysis can only be achieved by increasing the capacity of the separation column, which In turn, the volume of the equipment will become larger, which is not suitable for the operation and use in the laboratory environment. At the same time, the dynamic balance of the equipment is not easy to ensure after the volume becomes large, and the stability of the equipment is poor.

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