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A method for constructing an interstitial pneumonia model with autoimmune characteristics

A construction method and autoimmune technology, applied in animal husbandry and other fields, can solve problems such as injury, organic pathological changes of lung tissue, and difficulties in building animal models of interstitial pneumonia

Active Publication Date: 2021-11-30
凯斯艾生物科技(苏州)有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Although the disease is often accompanied by many systemic complications and lung damage, it does not show typical organic pathological changes of lung tissue
[0005] At present, there are still difficulties in the construction of animal models of interstitial pneumonia with autoimmune characteristics, and it is difficult for people to obtain ideal animal models with representative pathological changes for the study of interstitial pneumonia with autoimmune characteristics

Method used

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  • A method for constructing an interstitial pneumonia model with autoimmune characteristics
  • A method for constructing an interstitial pneumonia model with autoimmune characteristics
  • A method for constructing an interstitial pneumonia model with autoimmune characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Rat model of interstitial pneumonia with autoimmune features

[0028] Select 20 adult SD female (male) rats, weighing 270-300g, and randomly divide them into 4 groups, 12 h light and night cycle, temperature 22 ℃ ± 2 ℃, humidity 50%-60%. Grouped as follows:

[0029] Sham model group (normal animals, n=5), model-1 week group (n=5), model-2 week group (n=5), model-3 week group (n=5)

[0030] Model establishment: Animals in the model-1 week group, model-2 week group, and model-3 week group were anesthetized with isoflurane, intubated through the mouth with polyethylene PE10 trachea, entered the left bronchus, and perfused with TNBS sodium chloride solution; 1mL / kg (50mg / mL), and then fed normally. After 1 week, 2 weeks, and 3 weeks, the animals in each model group were dissected to observe the lung tissue, which was preserved in formalin solution.

[0031] Animal body weight changes in each model group ( figure 1 A) and percentage of body weight change ( fig...

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Abstract

The invention discloses a method for constructing an interstitial pneumonia model with autoimmune characteristics: adult rats are orotracheally intubated, TNBS sodium chloride solution is perfused into the bronchi, and then fed with common feed for 3-6 weeks. The rat model of interstitial pneumonia with autoimmune characteristics was constructed, with the pathological characteristics of immune inflammation of small bronchi and accompanying pulmonary arterioles and the pathological changes of alveolar tissue inflammation in the accumulated area, which can be used to screen for prevention or treatment of autoimmune diseases. Drugs for immunologically characterized interstitial pneumonia and as an animal model for the study of autoimmunely characterized interstitial pneumonia.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an interstitial pneumonia model with autoimmune characteristics and its construction method and application. Background technique [0002] Idiopathic interstitial pneumonia is a group of diseases characterized by diffuse inflammation and / or fibrosis in the lungs. Clinically, some patients with idiopathic interstitial pneumonia have autoimmune features, but they cannot be diagnosed as a specific connective tissue disease. In 2015, the European Respiratory Society and the American Thoracic Society recommended that it be named "interstitial pneumonia with autoimmune features (IPAF)". IPAF is similar to other interstitial lung diseases, but it has certain characteristics in terms of epidemiology, clinical manifestations, serological examination, morphological manifestations, lung function tests, other tests and prognosis. More importantly, the pathological analysis results of local lun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0368
Inventor 陈继巧杨祖经沈红英靳策庄永杰
Owner 凯斯艾生物科技(苏州)有限公司
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