A kind of diafiltration device and diafiltration process for pyrogen-free gene therapy supercoiled plasmid

Abstract

The invention discloses a pyrogen-free gene therapy supercoiled plasmid diafiltration device and diafiltration process, comprising a diafiltration device shell, a connecting component, a power supply box, a diafiltration device top cover, a fixing component, a power line tube, and an electrode block and a feed hole, the top of the filtering device shell is provided with a filtering device top cover, the top side of the filtering device top cover is fixed with a power supply box by screws, and the bottom side of the filtering device top cover is provided with a power supply The top of the power line tube is connected to the bottom of the power box, and the bottom of the power line tube is provided with an electrode block. The pyrogen-free gene therapy superhelical plasmid washing and filtering device has a simple structure and is easy to operate. It is clamped by rotating a semicircular splint The feed pipe on the outside of the inner liner prevents material leakage caused by the feed pipe outside the inner liner due to its own vibration or external impact, which is conducive to saving resources and facilitating processing. The feed tube is fixed.

Description

technical field [0001] The invention relates to the technical field of gene therapy, in particular to a diafiltration device and a diafiltration process for pyrogen-free gene therapy supercoiled plasmids. Background technique [0002] Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by defects and abnormal genes to achieve therapeutic purposes. It is inserted into the appropriate recipient cells of the patient so that the product produced by the exogenous gene can treat a certain disease. In gene therapy, it is necessary to wash and filter the non-pyrogenic supercoiled plasmid; it is used when the traditional supercoiled plasmid is used for washing and filtering Electrophoresis technology, the device used for diafiltration of supercoiled plasmids is generally integrated, and users cannot install and disassemble it by themselves. After a long time of use, raw materials are easily accumulated insid...

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Problems solved by technology

[0002] Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by defects and abnormal genes to achieve therapeutic purposes. It is inserted into the appropriate recipient cells of the patient, so that the product produced by the exogenous gene can treat a certain disease. In gene therapy, it is necessary to wash and filter the non-pyrogenic supercoiled plasmid; it is used when the traditional supercoiled plasmid is used for washing and filtering Electrophoresis technology, the device used for diafiltration of supercoiled plasmids is generally integrated, and users cannot install and disassemble it by themselves. After a long time of use, raw materials are easily accumulated inside the device, resulting in blockage of the device and serious damage to the device. At the same time, traditional supercoiled plasmids are used for diafiltration. The operation is complicated, wastes time and manpower, and is not conducive to processing and production; in view of these defects, it is necessary to design a diafiltration device and diafiltration process for pyrogen-free gene therapy supercoiled plasmids

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