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Biomarkers for diagnosis and/or prognosis evaluation of metastatic esophageal carcinoma

A prognostic assessment, esophageal cancer technology, applied in the biological field, can solve problems affecting the effect of tumor radiotherapy or chemotherapy

Inactive Publication Date: 2020-11-24
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A large number of studies have shown that the occurrence and development of tumors are accompanied by changes in miRNA levels, which affect the effect of radiotherapy or chemotherapy on tumors

Method used

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  • Biomarkers for diagnosis and/or prognosis evaluation of metastatic esophageal carcinoma
  • Biomarkers for diagnosis and/or prognosis evaluation of metastatic esophageal carcinoma
  • Biomarkers for diagnosis and/or prognosis evaluation of metastatic esophageal carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Materials and methods

[0054] clinical samples

[0055] A total of 10 patients with ESCC underwent routine surgery at the affiliated hospital from May to November 2018 at Changzhou Second People's Hospital of Nanjing Medical University in 2018. The tumor samples were extracted and preserved using liquid nitrogen cryopreservation until RNA extraction. This study was sponsored by Changzhou Second People's Hospital of Nanjing Medical University, and informed consent was issued.

[0056] cell culture

[0057] In this study, normal esophageal epithelial cell lines (HEEC) and esophageal squamous cell carcinoma cell lines (TE1, TE10-ECa-109, Kyse-30, Kyse-70) were purchased from the cell bank of Fudan University in Shanghai, and 10% fetal bovine serum (Invitrogen , Carlsbad, CA) and penicillin (100u / ml) were cultured in RPMI-1640 medium. Cells were incubated at 37°C and 5% carbon dioxide.

[0058] cell transfection

[0059] Specific siRNAs against KIF22 (siKIF22), sicram...

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Abstract

The invention discloses biomarkers for diagnosis and / or prognosis evaluation of metastatic esophageal carcinoma. The invention discloses an application of KIF22 and / or miR-122 as biomarkers in the preparation of reagents, kits or detection devices for diagnosis and / or prognosis evaluation of metastatic esophageal carcinoma. The invention discloses an application of KIF22 and / or miR-122 as therapeutic targets in the preparation of drugs for the treatment of metastatic esophageal carcinoma. The invention suggests that the interaction between miR-122 and KIF22 can be used as a new strategy for controlling the growth and transfer of ESSC cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biomarker for esophageal cancer metastasis diagnosis and / or prognosis assessment. Background technique [0002] Esophageal cancer (EC) is the 8th most frequently analyzed cancer and the 6th most common cause of cancer-related death in the world. Esophageal cancer includes two histological types, esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC). In China, there are more than 100 ESCC cases per year within 100,000 people. Although ESCC has progressed rapidly with chemotherapy, radiotherapy, and esophagectomy, the 5-year survival rate remains around 14% due to tumor metastasis. Therefore, elucidating the underlying mechanism of tumor development will help to understand the research progress of ESCC in my country. [0003] Kinesin superfamily proteins (KIFs) are microtubule-dependent molecular engine proteins that transport organelles, protein complexes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K45/00A61P35/04
CPCA61K45/00A61P35/04C12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158G01N33/57407G01N33/57484G01N33/68G01N2333/4703
Inventor 汪建林马承贤朱宏明孙菲
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL