[0054] Example 2: Such as Figure 5-10 As shown, the sampling device includes a sampling device body 7, a five-piece cup 1, a ball plunger 3, and a driving device 4. The ball plunger 3 is connected to the sampling device body 7 In the above, the driving device 4 drives and controls the movement of the five-piece cup 1; the ball plunger 3 limits the position of the five-piece cup 1; the sampling device also includes a hand shifting device 5, The hand device 5 includes a second shifting hand 501, a first shifting hand 502, and a connecting rod 503. The second shifting hand 501 and the first shifting hand 502 are respectively arranged at both ends of the connecting rod 503. One side of a shifting hand 502 is provided with a shifting hand shaft 506; the connecting rod 503 is connected with a spring 504 under the first shifting hand 502 through a spring fixing block 505, one end of the spring 504 is connected to the first The shifting hand 502 is in pressing contact and the optocoupler limit piece 6 is connected under the other end of the spring 504; the driving device 4 drives the five-piece cup 1 to move through the shifting device 5; The hand device 5 enables the driving device 4 to use the second shifting hand 501 and the first shifting hand 502 to drive the five-piece cup 1 to move, and at the same time, the second shifting hand 501 and the first shifting hand 502 also have a position limiting effect on the five-piece cup 1, thereby Better control of the movement and position of the five-piece cup 1; setting the photocoupler limit piece 6 can prompt the position of the dial device; the driving device 4 includes a fixed block 405 and a guide 402, the fixed block 405 One side is engaged with the guide rail 402 to make the fixed block 405 move on the guide rail 402; the shifting device 5 is fixed on the fixed block 405, and the fixed block 405 drives the shifting The hand device 5 moves along the guide rail 502; the five-piece cup 1 is placed between the second shifting hand 501 and the first shifting hand 502 and is controlled by the second shifting hand 501 and the first shifting hand 501 The hand 502 drives the quintuple 1 to move; the fixed block 405 and the guide rail 402 are used to drive the toggle device 5 to move along the rail 402, so that the quintuple 1 moves back and forth along the guide rail 402, which is beneficial to better control the quintuple 1 movement; the sampling device body 7 includes the first support column 701, the second support column 702, the first side baffle 703 and the second side baffle 704; the first side baffle 703 and the The second side baffle 704 is arranged in parallel and connected between the first support post 701 and the second support post 702; the ball plunger 3 is installed on the first side baffle 703; The first side baffle 703 and the second side baffle 704 are arranged to set the shifting device 5 and the quintuple 1 between the first side baffle 703 and the second side baffle 704, and the lower end of the shifting device 5 is exposed The underside of the first side baffle 703 and the second side plate 704 are fixed on the fixed block 405 outside the second side baffle 704; the side of the ball plunger 3 is connected with a contact sensor through a fixed compression connecting block 8 The probe 9, the fixed compression connecting block 8 is installed on the outside of the first side baffle 703 and connects the ball head The plunger 3 is enclosed in the space of the fixed compression connecting block 8; the driving device 4 is installed on the outside of the second side baffle 704, and the driving device 4 further includes a motor 403, a screw rod 404 and a sliding Block 401, the motor 403 is connected to the screw 404, the slider 401 is sleeved on the screw 404, the slider 401 is connected to the fixed block 405, and the guide rail 402 is installed On the outside of the second side baffle 704; the sampling device further includes a code scanner 10, the code scanner 10 is installed on the outside of the first side baffle 703 and installed on the fixed compression connection One side of the block 8; a code scanner 10 is provided for inputting information about the sample to be tested in the five-piece cup 1; the sampling device also includes a guide groove 2 and a sampling slot vacancy 201, the sampling slot vacancy 201 is used to place the pre-discarded quintuple cup 1, the quintuple cup 1 is driven to slide in the guide groove 2 to the vacancy position 201 of the sampling slot by the dialing device 5; the sampling device also includes The optocoupler 11 is arranged on the first support column 701 through the optocoupler support 12 and cooperates with the optocoupler limiting sheet 6 for prompting the position of the dial device 5; The second side baffle 704 is provided with notches, and the notches are connected by a connecting block 705. The five-piece cup 1 includes a connecting sleeve 102 and five cup grooves 101. The connecting sleeve 102 is provided with five connecting holes 103 corresponding to the cup groove 101. The five cup grooves 101 and the five cup grooves 101 respectively The connecting holes 103 are vertically connected in a one-to-one correspondence, so that a plurality of the cup grooves 101 are independent of each other; the five-piece cup 1 further includes a cup sleeve 104 that surrounds the outer walls of the five cup grooves 101 and The connecting sleeve 102 is vertically connected; the connecting sleeve 102 also includes a number of connecting portions 105, the connecting portions 105 are respectively arranged between the five cup grooves 101 and the cup groove 101 and the cup sleeve 104 Between; one end of the connecting sleeve 102 extends in the horizontal direction with a handle 106; the outer edge of the handle 106 is arc-shaped, the back of the handle 106 is provided with 4 anti-skid strips 107; the five The cup 1 also includes two reaction cups 108, and the reaction cup 108 is placed in the cup groove 101; the end of the quintuple 1 away from the handle 106 is provided with a card groove 109 on the connecting sleeve 102; The cup groove 101 of the quintuple 1 is divided into a No. 1 cup groove, a No. 2 cup groove, a No. 3 cup groove, a No. 4 cup groove, and a No. 5 cup groove, and the card groove 109 is located in the No. 1 cup groove and Between the second cup groove; the diameter of the second cup groove and the fifth cup groove is larger than the first cup groove, the third cup groove, and the fourth cup groove, the second cup groove and the The fifth cup groove is used to place the reaction cup 108; wherein the five cup 1 has a groove 109 between the first cup groove and the second cup groove, which is engaged with the spherical plunger 3 and is used for the five cup The limit of 1; the second cup slot in the quintuple 1 contains the reagent reaction cup, and the reagent reaction cup is designed to be protected from light and contains the labeled antibody; the reaction cup 108 is placed in the fifth cup slot, which is the sample reaction to be tested The other cups and troughs directly load the reagents into them. This setting realizes that all the reagents required for the test are integrated into a test cartridge, which is convenient to use and can save clinical test time; the five-piece cup 1 is a single consumable. It can effectively avoid the contamination that may be caused by repeated cleaning and use of the sample holder, thereby improving the detection accuracy.
[0055] The sample injection process of the sample injection device is as follows: firstly place the reagent reaction cup in the second cup slot in the quintuple 1 and contain the labeled antibody; put the reaction cup in the fifth cup slot, which is the sample reaction cup to be tested, other cups and slots Load the reagent directly into it; the quintuple cup 1 is scanned by the code scanner 10, and the information of the sample to be tested is entered, and then the quintuple cup 1 is put into the sample inlet of the testing instrument, the motor 403 is started, and the motor 403 passes through the screw rod 404 drives the sliding block 401 to move, and the sliding block 401 drives the fixed block 405 to move on the guide rail 402. Synchronously, the fixed block 405 drives the hand setting device 5 connected to it to move along the guide rail 402, thereby driving the quintuple 1 to move together; The front end of the quintuple 1 reaches the spherical plunger 3, and the slot 109 at the front end of the quintuple 1 engages with the spherical plunger 3, prompting to reach the manual placement position; the contact sensor probe 9 senses the quintuple 1 Position; at this time, the first shifting hand 502 is in the initial position, and in the corner of the first support column 701, the second shifting hand 501 touches the front end of the quintuplet 1, and the detection instrument display prompts that the next operation can be performed; After the labeled antibody, buffer, sample, magnetic beads, etc. in the quintuple cup 1 are mixed, the sample reaction cup and the labeled antibody cup are transferred to the incubation plate; the testing instrument controls the quintuple cup 1 to be moved forward by the dial device 5, Under the action of the spring 504 contraction of the first shifting hand 502 and the shifting hand shaft 506, the head of the first shifting hand 502 is tilted and jams the lower end corner of the holding part 106 of the quintuple 1 to drive the quintuple 1 to move. The union cup 1 slides in the guide groove 2, and when it reaches the vacant position 201 of the sampling slot, it passes downwards through the waste tank to discharge the testing instrument; after the quintuple cup 1 is discarded, the movement of the motor 403 drives the shifting device 5 forward. The photocoupler 11 on the support column 701 cooperates with the photocoupler limit piece 6 fixedly connected to the first shifting hand 502, prompting the shifting device 5 to reach the initial position and can enter the quintuple 1 again.
[0056] Such as Picture 11 As shown, the method of using five-piece cups to test samples includes the following steps:
[0057] (1) The five-piece cup receiving device is ejected from the sampling port of the testing instrument;
[0058] (2) Put the five-piece cup 1 in the receiving device and add the sample to be tested into the sample reaction cup into the fifth cup slot, and add the labeled antibody to the reagent reaction cup into the second cup slot, and at the same time the first cup slot The labeling antibody buffer is preset inside, the reaction buffer is preset in the third cup slot, and the fourth cup is preset with magnetic beads;
[0059] (3) The Wulian Cup 1 enters from the sampling port of the detection instrument;
[0060] (4) First, use the combined cleaning needle to suck the labeled antibody buffer in the No. 1 cup, add it to the labeled antibody in the reagent reaction cup, and mix;
[0061] (5) After cleaning the combined cleaning needle, suck the reaction buffer in the third cup tank, add it to the fourth cup tank, mix it with the magnetic beads in the fourth cup tank, and then absorb the mixed liquid;
[0062] (6) Add the mixed liquid in step (5) to the sample reaction cup and mix;
[0063] (7) Grab the reagent reaction cup and the sample reaction cup into the turntable to incubate the sample reaction cup;
[0064] (8) After cleaning the combined cleaning needle, suck the liquid in the reagent reaction cup on the turntable and flush it into the sample reaction cup, mix it, and then incubate;
[0065] (9) Wash the combined cleaning needle, move the sample reaction cup into the dark room, add liquid to develop color and read the result.
