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Sudden cardiac death susceptibility detection kit based on hspa1b gene indel polymorphism

A detection kit and sudden cardiac death technology, applied in the field of detection, can solve the problem of not predicting the susceptibility of SCD

Active Publication Date: 2022-03-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, there is no research report on the correlation between the rs3036297 indel polymorphism and SCD, and there is no related report on predicting the susceptibility to SCD by detecting the indel polymorphism site in the 3'UTR of the HSPA1B gene

Method used

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  • Sudden cardiac death susceptibility detection kit based on hspa1b gene indel polymorphism
  • Sudden cardiac death susceptibility detection kit based on hspa1b gene indel polymorphism

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Embodiment 1

[0025] Embodiment 1: the use of detection kit

[0026] Step 1: Extraction of DNA template

[0027] Genomic DNA from peripheral blood was extracted using a blood genomic DNA extraction system (non-centrifugal column type).

[0028] Step 2: PCR reaction - replication of the target fragment

[0029] Use a PCR assay kit for SCD susceptibility, which contains the following primers:

[0030] SEQ ID No.1: 5'-ACTGTTGGGACTCAAGGACT-3', the Tm value is 58°C;

[0031] SEQ ID No.2: 5'-TACAAAAATAATGAAGCCAGCTAAT-3', the Tm value is 56°C;

[0032] The pair of primers can specifically amplify the fragment containing the polymorphism of the rs3036297 insertion-deletion site in the HSPA1B gene.

[0033] The total volume of the PCR reaction system is 10ul, including: 1μl DNA template, 0.04μl each of two 50μM specific primer pairs, 0.08μl 2.5U / μl Taq DNA polymerase; 0.2μl 2.5mM dNTP mixture; 25mM MgCl 2 Solution 0.6 μl; 10×PCR reaction buffer 1 μl; make up with deionized water; react on the E...

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Abstract

The invention discloses a susceptibility detection kit for sudden cardiac death based on the insertion-deletion polymorphism site of HSPA1B gene. The specific primer pair contained in the kit of the present invention is designed for the rs3036297 insertion-deletion site on the HSPA1B gene, which can specifically amplify the DNA fragment containing this site and detect fragments of different lengths in capillary electrophoresis Combined with the findings of our case-control study, it can be considered that those carrying deletion alleles at the rs3036297 indel site on the HSPA1B gene of the detected DNA are susceptible to SCD. Therefore, this technology can predict the susceptibility of individual SCD by detecting the genotype of the rs3036297 indel site on the HSPA1B gene of the individual.

Description

technical field [0001] The invention relates to a sudden cardiac death susceptibility detection kit based on an insertion-deletion polymorphism site of HSPA1B gene, which belongs to the technical field of detection. Background technique [0002] Sudden cardiac death (sudden cardiac death, SCD) refers to sudden unexpected death caused by cardiac causes, manifested as sudden loss of consciousness and sudden circulatory and respiratory arrest in a short period of time. "Short-term" is defined as less than 1 hour in the case of witnesses, and 24 hours in the absence of witnesses. Epidemiology shows that 40.7 people per 100,000 people die from SCD every year in China. Considering the huge population base in my country, the number of SCD cases is very large. A large number of previous studies have shown that the cause of death in SCD is mostly coronary atherosclerotic heart disease or fatal arrhythmia. The former is more common in elderly people, while the latter is more common ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2563/107
Inventor 何艳杨真真陈雪坤于欢高玉振
Owner SUZHOU UNIV