Fluorescence microendoscope for microscopy multispectral imaging

Abstract

The invention provides a fluorescence microendoscope for microscopy multispectral imaging. The fluorescence microendoscope comprises a C threaded opening, a collimating mirror, a light splitting element, an imaging mirror and an area array detector which are arranged in the preset axis direction. The collimating mirror and the imaging mirror are separately arranged at two sides of the light splitting element and are arranged in a bilateral symmetry manner corresponding to the center of the light splitting element, the C threaded port is located at the left side of the collimating mirror, and the area array detector is located at the right side of the imaging mirror. The fluorescence microendoscope has the beneficial effects that the static spectral imager host adopts the C threaded port and can be connected with different types of microscopes through the C conversion interface, so that different types of microscopic spectral imaging systems are realized; the light splitting element isarranged in the spectral imager host, and the influence of ambient light on the light splitting effect is small.

Description

technical field [0001] The invention relates to a fluorescent microendoscope for microscopic multispectral imaging, belongs to the technical field of optical instruments, and is applied to the fields of biomedicine, material science, forensic science, and microelectronic technology. Background technique [0002] Ordinary optical microscopes have always been a basic tool for human beings to study tiny objects. Although they have reached a very high level in terms of resolution and imaging quality, they can only observe and analyze the shape of samples, and cannot obtain information related to the structure and quality of samples. Further detailed information related to chemical composition has been unable to meet the growing needs of practical applications. [0003] Spectral imaging technology can not only obtain the spatial information of the target, but also obtain the spectral information of each point of the target. Due to the different structure and chemical composition...

AI Technical Summary

Problems solved by technology

In the actual use process, this push-broom method brings two problems: one is that push-broom is a mechanical movement, the speed is relatively slow, and the image acquisition time is longer; the other is that push-broom movement makes the operation cumbersome and reliability Not high enough to be used as a general purpose device

Chinese patent application number: 200810036683.0, "Molecular Spectral Imager" uses Acousto Optic Tunable Filter (AOTF) or liquid crystal tunable filter as the dispersion unit, which avoids push-broom movement and can achieve fast imaging. However, The system realizes spectral imaging by splitting the light source, and the measurement will be affected by ambient stray light, which will adversely affect the imaging quality

Similar to the "Molecular Spectral Imager", it also achieves spectral imaging by splitting the light source. The measurement will be affected by ambient stray light, which will adversely affect the imaging quality.

Chinese patent application number: 201310200049.7, "A Microscopic Spectral Imaging Device and Method Based on Liquid Crystal Filter Device" discloses a microscopic spectral imaging device based on a liquid crystal filter device (LCTF), including CCD, lens barrel, and imaging lens assembly , LCTF, and microscope objective lens are located on the same optical axis, the imaging lens is located inside the lens barrel, and the LCTF is located between the lens barrel and the microscope objective lens. microscopic device

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