Application of Lactobacillus johnsonii in the prevention and/or treatment of inflammatory bowel disease

A technology for Lactobacillus johnsonii and inflammatory bowel disease, applied in the field of oral microecological preparations, can solve problems such as insignificant curative effect, and achieve the effect of improving symptoms

Active Publication Date: 2022-08-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that a variety of probiotics such as lactic acid bacteria and bifidobacteria can alleviate inflammatory bowel disease, but there are still some disadvantages, such as the curative effect is not obvious

Method used

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  • Application of Lactobacillus johnsonii in the prevention and/or treatment of inflammatory bowel disease
  • Application of Lactobacillus johnsonii in the prevention and/or treatment of inflammatory bowel disease
  • Application of Lactobacillus johnsonii in the prevention and/or treatment of inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Isolation, identification and culture of Lactobacillus johnsonii

[0061] 1.1. Isolation of Lactobacillus johnsonii

[0062] The mouse colonic chyme was placed in a 1.5 ml centrifuge tube, 500 μl of PBS was added, and vortexed for 1 minute to completely suspend it. The obtained suspension was plated on MRS (Man, Rogosa and Sharpe) agar plate with 10-fold serial serial dilution, and cultured at 37°C overnight.

[0063] 1.2. Identification of Lactobacillus johnsonii

[0064] Pick the monoclone of described Lactobacillus johnsonii in MRS liquid medium, amplify the full length of 16S rDNA after static culture, and the primer sequences are respectively:

[0065] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0066] 1492R: 5'-CGGYTACCTTGTTACGACTT-3'.

[0067] The PCR reaction system is shown in Table 1:

[0068] Table 1. PCR reaction system

[0069]

[0070] PCR reaction conditions are as follows:

[0071]

[0072] The PCR products obtained were detected by 1% aga...

Embodiment 2

[0076] Example 2. Prevention and treatment of inflammatory bowel disease mice by Lactobacillus johnsonii

[0077] 2.1. Cultivation of Citrobacter

[0078] Citrobacter bacillus (DBS100) was activated twice and then inoculated into a conical flask containing 50 mL of LB (Luria-Bertani) medium, sealed to form a microaerobic environment, and cultured in a constant temperature shaker at 37°C for 12 hours at 220 rpm for later use.

[0079] 2.2. Establishment, grouping and administration of inflammatory bowel disease mouse model

[0080] After a one-week acclimation period, mice were randomly divided into 4 groups of 12 mice based on body weight, including:

[0081] Control group: the mice in this group were given PBS every 2 days;

[0082] Sick group (i.e. Citrobacter gavage group): The mice in this group were given PBS every 2 days, and 2×10 were given on the 8th day. 9 CFU of Citrobacter, and then continued to feed for a week, in which PBS was administered every 2 days;

[008...

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Abstract

The invention belongs to the field of biomedicine, and discloses the application of Lactobacillus johnsonii in the preparation of medicaments for preventing and / or treating inflammatory bowel disease, and a method for preventing and / or treating inflammatory bowel disease of oral probiotics comprising Lactobacillus johnsonii strains such as UMNLJ22. In the present invention, Lactobacillus johnsonii can significantly improve spleen hyperplasia, colon shortening, colonic mucosal hyperplasia and colon inflammatory response in the inflammatory bowel disease mouse model induced by Citrobacter rodentium, and it is found that Lactobacillus johnsonii can significantly Alleviate inflammatory bowel disease in mice, so as to be used for the development and preparation of medicaments for preventing and / or treating inflammatory bowel disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of Lactobacillus johnsonii in the preparation of medicaments for preventing and / or treating inflammatory bowel disease and an oral microecological preparation comprising the Lactobacillus johnsonii. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic, relapsing inflammatory bowel disease. Clinical symptoms are mainly abdominal pain, abdominal distension, diarrhea and blood in the stool. In the past few decades, the global incidence of inflammatory bowel disease has increased year by year, which makes inflammatory bowel disease a major public health problem worldwide. [0003] Although the research on inflammatory bowel disease has become more complete and systematic in recent years, the pathogenesis and pathogenesis of inflammatory bowel disease are still unclear. Studies in recent years have shown that the factors affecting inflammatory bowel di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225
CPCA61K35/747A61P1/00A61P29/00A61P1/04C12N1/20A61K31/60C12R2001/225C12N1/205A61K2300/00Y02A50/30
Inventor 武振龙张云常杨鹰
Owner CHINA AGRI UNIV
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