Microfluidic radio frequency probe for detecting trace marker

Abstract

The invention discloses a microfluidic radio frequency probe for trace marker detection. The probe comprises an interface panel and a cylindrical protective shell connected with the interface panel, aprobe main body is arranged in the protective shell, and the probe main body comprises a sample detection module, a matching circuit module, a gas-liquid exchange module and a support structural member which are arranged in sequence. The invention provides a one-stop detection method and an implementation scheme integrating sample pretreatment and detection for low-concentration and small-amountsamples. Through the combination of different modules, the detection of some trace marker samples in various states can be realized.

Description

technical field [0001] The invention relates to the field of magnetic resonance spectroscopy, in particular to a microfluidic radio frequency probe for detecting trace markers. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In recent years, among all types of cancer, the incidence and mortality of lung cancer have increased year by year, and it has become the "number one killer" of cancer patients in my country. Therefore, the "early diagnosis" and "early treatment" of lung cancer are particularly critical. In terms of pathology, lung cancer can be roughly divided into two types: small cell lung cancer and non-small cell lung cancer, and can be further divided into more types. Because different types of lung cancer should be treated with different treatment plans, the early classification method of lung cancer has very important cl...

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Problems solved by technology

This method has the following deficiencies: 1. The waiting time for the ventilation step is long, and the ventilation step often accounts for more than half of the total time in actual operation, and in this scheme, due to the generation of air bubbles due to ventilation, the static magnetic field intensity will be uneven. Therefore, this step cannot be paralleled with the other two steps; 2. Bubble vibration will be generated when the sample in the detection area is directly ventilated, which will cause some sample solutions (such as charged colloids) to coagulate, which will lead to failure to measure the ideal spectrum; 3. Air bubbles will occur in some amphiphilic samples (phospholipids, etc.), causing the position of the sample to deviate from the detection area. At this time, adding a defoamer will affect the properties of the sample itself

In the existing literature, the parahydrogen-induced nuclear polarization enhanced H-1NMR method can be used by reducing the size of the coil and integrating the chip in the probe, but its sensitivity is low, and the concentration of substances in μmol / L can only be observed after hundreds of times of accumulation. signal, while the background signal is large and complex, and it is difficult to distinguish the signal of a single substance

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