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SNP Molecular Markers of Genes Related to Wheat Grain Weight and Its Application

A technology of wheat and genotyping, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., and can solve problems such as the excellent natural allelic variation of TaFLO2-2A gene coding sequence.

Active Publication Date: 2022-05-10
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, there is no SNP site and excellent natural allelic variation in the TaFLO2-2A gene coding sequence reported in wheat

Method used

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  • SNP Molecular Markers of Genes Related to Wheat Grain Weight and Its Application
  • SNP Molecular Markers of Genes Related to Wheat Grain Weight and Its Application
  • SNP Molecular Markers of Genes Related to Wheat Grain Weight and Its Application

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Embodiment 1

[0076] Wheat YZ4110 (National Audited Wheat 2003032, which can be obtained from the seed industry company) is one of the main wheat varieties in the Huanghuai winter wheat region of my country, and wheat MY5122 is an excellent wheat material introduced from abroad (can be obtained from the Crop Germplasm Resource Bank of the Chinese Academy of Agricultural Sciences, and the unified number MY005122). Fresh leaves of these two wheat materials were taken, ground with liquid nitrogen and total RNA was extracted, and the RNA was reverse transcribed. The gene sequences of three homologous genes of Chinese spring wheat TaFLO2-2A (TraesCS2A01G517100.3), TaFLO2-2B (TraesCS2B02G545600.2), and TaFLO2-2D (TraesCS2D02G518700.2) can be downloaded from the China Spring Reference Genome Sequence website. In the comparison of the three homologous genes, genome-specific primers can be designed according to the SNP differences among the three homologous genes to specifically amplify the TaFLO2-2A...

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Abstract

The present invention relates to a SNP molecular marker of a wheat grain weight-related gene and its application. The wheat SNP site is used in the identification or auxiliary identification of wheat thousand-grain weight traits. The wheat SNP site is located in TaFLO2-2A of Chinese spring wheat The 2209th position of the gene coding region, the nucleotide of the SNP site is C or T. The invention can realize the screening of high-grain weight wheat and provide guidance for the subsequent cultivation and breeding of wheat.

Description

technical field [0001] The invention relates to a SNP molecular marker of a wheat grain weight-related gene and an application thereof, in particular to a molecular marker for detecting the natural allelic variation of the coding region of the wheat grain weight gene TaFLO2-2A and the application thereof. Background technique [0002] Wheat is one of the three major food crops in the world, how to further increase the yield of wheat has important production practical significance. Common wheat, bread wheat (Triticum aestivum) is an allohexaploid wheat (AABBDD) formed by natural hybridization and chromosome doubling of tetraploid wheat Triticum turgidum (AABB) and diploid goat grass Aegilops tauschii (DD) . Due to its huge genome, the research on the relationship between yield and grain weight lags behind other crops for a long time. Recently, with the publication of the wheat genome reference sequence (IWGSC, 2018), new and effective tools have been provided for further an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 程佑发苏熙涵郭志爱贾继增
Owner INST OF BOTANY CHINESE ACAD OF SCI
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