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Flexible electrode for simultaneously detecting hypochlorous acid and ascorbic acid, preparation method and application thereof

A technology of ascorbic acid and flexible sensors, which can be used in measuring devices, electrochemical variables of materials, and material analysis through electromagnetic means, etc. It can solve the problem of simultaneous detection of target analytes, lack of surface functionalization of flexible sensors, and can only measure the amount of change and other problems, to achieve the effect of improving detection accuracy, simple design, and avoiding measurement errors

Inactive Publication Date: 2021-01-08
SHANGQIU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most current electrochemical sensors designed for HClO or AA detection have the following challenges: (1) The oxidation and reduction potentials of the electrochemical determination relative to the reference electrode are affected by the difference between the internal and external environments, and the control potential measurement The accurate quantification of actual samples is limited by this method, and often only the amount of change can be measured; (2) the interference between target analytes will have a negative impact on their simultaneous detection; (3) the surface functionalization of flexible sensors is still lacking, and the application limited range

Method used

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  • Flexible electrode for simultaneously detecting hypochlorous acid and ascorbic acid, preparation method and application thereof
  • Flexible electrode for simultaneously detecting hypochlorous acid and ascorbic acid, preparation method and application thereof
  • Flexible electrode for simultaneously detecting hypochlorous acid and ascorbic acid, preparation method and application thereof

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Embodiment 1

[0040]The present invention will be further described in detail with reference to the following specific examples and drawings. Except for the content specifically mentioned below, all are common knowledge and common knowledge in the field, and the present invention does not specifically limit the content. Example 1: Simultaneous analysis of flexible sensor preparation

[0041](1) HClO specific probe synthesis

[0042]First, mix methylene blue (1.12g, 3.75mmol) and Na2CO3(1.59 g, 15.00 mmol) dissolved in 5 mL of water, then dichloromethane (10 mL) was added, and then the mixture was stirred at 40°C under nitrogen. Then sodium dithionite (2.61 g, 15.00 mmol) was dissolved in 5 mL of water and injected directly into the solution using a syringe. After the addition was complete, the mixture was stirred at 40°C under nitrogen until the solution turned yellow (30 min). The dichloromethane layer was separated from the aqueous layer and quickly dried over anhydrous sodium sulfate. After the sodi...

Embodiment 2

[0047](d) Immerse the GP / ERGO-CNT electrode in a solution of MBS and AQ (molar ratio 1:1), modify it with π-π bond for 10 minutes, take it out, and wash it with ethanol and distilled water 3 times in turn to obtain GP / ERGO- CNT / AQ+MBS; Example 2: Electrochemical characterization of flexible sensor GP / ERGO-CNT / AQ+MBS

[0048]According to the design, the prepared flexible sensor GP / ERGO-CNT / AQ+MBS is used as the working electrode, Ag / AgCl is used as the reference electrode, and the Pt wire is used as the counter electrode. The DPV response of different modified electrodes in 0.1M PBS solution is measured. Potential window: -0.7~0.2V, the measuring principle of this sensor is as followsfigure 1 , The measurement result is asfigure 2 As shown, no peak is observed on the bare GP (figure 2 , Curve a); after modified GO-CNT, a small oxidation peak appears at -0.22 V (curve b), which may be attributed to the oxygen-containing functional groups on the GO surface; when the GP / GO-CNT electrode is...

Embodiment 3

[0049]Example 3: Sensor response to HClO

[0050]Using the prepared flexible sensor GP / ERGO-CNT / AQ+MBS as the working electrode, Ag / AgCl as the reference electrode, and Pt wire as the counter electrode, the DPV response after adding different concentrations of HClO to 0.1M PBS solution was measured. Potential window: -0.7~0.2V. When the concentration of HClO increases from 0.5μM to 50μM, an oxidation peak appears in the DVP curve of the GP / ERGO-CNT / AQ+MBS electrode. The peak current density at -0.25V gradually increased, while the peak current density at -0.55V remained almost constant (image 3 In A), the oxidation peak at -0.25V is selected as the detection signal, and the oxidation peak at -0.55V is used as the reference signal. In terms of peak current density ratio (jMB / jAQ ) Draw a calibration curve with HClO concentration. It is found that there is a good linear relationship with the concentration of HClO in the range of 0-20 μM. The linear equation is jMB / jAQ =0.1458CHClO (μM)...

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Abstract

The invention discloses a flexible electrode for simultaneously detecting hypochlorous acid (HClO) and ascorbic acid (AA), a preparation method and application thereof, and belongs to the technical field of application of carbon nanomaterials in biosensing. The preparation method comprises the following steps: preparing a graphene-carbon nanotube composite material (ERGO-CNT), adsorbing the graphene carbon nanotube composite material (ERGO-CNT) on the surface of flexible graphene paper (GP) through pi-pi accumulation, and carrying out electrochemical reduction to obtain GP / ERGO-CNT, and then modifying the surface of the GP / ERGO-CNT with a synthesized hypochlorous acid electrochemical specific recognition probe (MBS) and an internal reference molecule (AQ) to obtain the flexible sensor GP / ERGO-CNT / AQ+MBS capable of simultaneously detecting the HClO and the AA. The sensor is simple and rapid to prepare, has high accuracy and wide linear range for simultaneous determination of HClO and AA, can achieve simultaneous analysis and detection of HClO and AA in a trace amount of body fluid, and has a good application prospect.

Description

Technical field[0001]The invention belongs to the technical field of application of carbon materials in biosensing, and relates to a flexible sensor for simultaneous analysis of HClO and AA, and a preparation method and application thereof.Background technique[0002]Cellular redox homeostasis is essential for many physiological and pathological processes. Reactive oxygen species (ROS) are an important type of oxidant in cell signaling pathways, which participate in the entire process from cellular immune response to metabolism. Hypochlorous acid (HClO / ClO-) Is a kind of ROS with antibacterial activity, which is catalyzed by the endogenous production of hydrogen peroxide and chloride ions by myeloperoxidase in white blood cells. High intracellular HClO concentration can easily lead to severe tissue damage and can lead to neurodegeneration, inflammatory diseases, cardiovascular diseases and cancer. Ascorbic acid (AA), commonly known as vitamin C, is abundant in many fruits and vegetabl...

Claims

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Application Information

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IPC IPC(8): C07D279/30G01N27/327G01N27/48
CPCC07D279/30G01N27/3278G01N27/3277G01N27/48
Inventor 董辉赵乐周艳丽刘澜涛张银堂徐茂田
Owner SHANGQIU NORMAL UNIVERSITY
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