Methods and system for stimulating root exudation in plants
A plant and root technology, applied in the field of novel compounds and their combinations, can solve problems such as inability to distinguish plant molecules from other organisms
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Embodiment 1
[0176] Example 1. Method of inducing plants to produce higher amounts of exudates or metabolites using divided roots.
[0177]In the split-root technique of the present invention, the two root systems of a plant are physically separated such that one root system (root A) can be stimulated and the root system induced systematically by either the stimulated root system (root A) or by the unaffected root system. The stimulated root system (root B) or both secrete valuable exudates or valuable metabolites or intermediates. In addition, the aerial parts of the same plants can be used as stimulation sites or alternatively as sites for harvesting metabolites after induction of the root system.
[0178] In this example, the root system of a seedling plant is divided into two equal parts, and each of the two root system parts is then placed in one of the two divided root compartments, each containing plant medium or direct In separate hydroponic or aeroponic containers, each feeds wit...
Embodiment 2
[0181] Example 2. Method of Inducing Plants to Produce Higher Amounts of Exudates or Metabolites Using Divided Roots Obtained by Regeneration
[0182] In the split-root technique of the present invention, the two root systems of a plant are physically separated such that one root system is stimulated (root A) and the secretion of valuable metabolites from the second root system (root B) is systematically induced. product. In addition, the aerial parts of the same plants can be used as stimulation sites or alternatively as sites for metabolite harvesting after induction of the root system. In addition, local induced secretion was also obtained from root A.
[0183] Divided roots are produced by cutting off the main root, then regenerating the lateral roots until they are long enough to place the lateral roots directly in individual hydroponic or aeroponic containers. Each container was fed with plant medium.
[0184] Following stimulation, the plants are incubated for the de...
Embodiment 3
[0186] Example 3: Method of inducing plants to produce higher amounts of exudates or metabolites using alternatively obtained divided roots.
[0187] In the split-root technique of the present invention, the two root systems of a plant are physically separated such that one root system (root A) is stimulated and systematically induced to secrete valuable metabolites from the second root system (root B). product. Additionally, the aerial parts of the same plants can be used as stimulation sites or alternatively as sites for metabolite harvesting after induction of the root system. In addition, local induced secretion was also obtained from root A.
[0188] The root structure is modified by one or more of various means to generate more root surface space, for example, by increasing branching or total root biomass. Root structure can be altered by one or more of: 1) genetic engineering; 2) genome editing; 3) chemical or radiation mutagenesis; 4) identification of natural variat...
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