High-speed evanescent field frequency shift super-resolution microscopic imaging system and imaging method

Abstract

The invention discloses a high-speed evanescent field frequency shift super-resolution microscopic imaging system and imaging method, and the method comprises the steps: employing an on-chip light-emitting device to generate evanescent fields with three wavelengths transmitted in the same direction at the same time, and employing a color camera to collect a frequency shift image generated by the three-wavelength evanescent fields which illuminate in the same direction at the same time, wherein the three wavelengths correspond to frequency shift signals of three wave bands of red, green and blue, and the illumination of each wavelength has different frequency shift amounts. In the image reconstruction process, multi-wavelength information shot at the same time is separated out to be subjected to interpolation processing, and therefore sample information with different frequency shift amounts and different illumination directions is obtained. And finally, image reconstruction is carriedout, high-frequency and low-frequency spectrums of the sample in different directions are spliced by using a splicing algorithm in a spectrum space, and finally, a high-resolution image breaking through the optical diffraction limit of the traditional microscope is recovered. According to the invention, under the condition of sacrificing a certain image sampling rate, the image acquisition quantity of the evanescent field frequency shift super-resolution method can be effectively reduced, and the imaging speed is improved.

Description

technical field [0001] The invention relates to the field of super-resolution microscopy, in particular to a high-speed evanescent field frequency shift super-resolution microscopic imaging system and imaging method. Background technique [0002] The traditional optical system is limited by Abbe's diffraction limit, and its imaging resolution has a certain limit. In order to break through the resolution limit of the optical microscopy system, a series of fluorescent labeling and non-fluorescent labeling methods have been proposed. Among them, the frequency-shifting super-resolution microscopy imaging technology breaks the bandwidth limitation of traditional imaging systems and detector devices, and shows the advantages of fast and large field of view imaging. Frequency-shifting label-free super-resolution microscopy does not require fluorescent labels, and can achieve super-resolution imaging of non-biological samples, which has broad application prospects. [0003] The ev...

AI Technical Summary

Problems solved by technology

However, each image acquisition process requires a certain camera integration time, which limits the imaging speed of this imaging method and is not conducive to obtaining dynamic images with high frame rates.

Images