Polypeptide for detecting Seneca virus antibody and application of polypeptide

Abstract

The invention discloses a polypeptide for detecting a Seneca virus antibody and application of the polypeptide. The polypeptide is composed of an eVP1-1 and an eVP2-1. The eVP1-1 is a polypeptide of P11 or P12: P11, wherein the P11 is a polypeptide of which the amino acid sequence is SEQ ID No. 1, and the P12 is a polypeptide of which the amino acid sequence is the 2-16th site of SEQ ID No. 1; theeVP2-1 is a polypeptide of P21 or P22, wherein the P21 is a polypeptide of which the amino acid sequence is SEQ ID No.2, and the P22 is a polypeptide of which the amino acid sequence is the 2-15th site of SEQ ID No.2. The antibody detection kit prepared from the polypeptide is high in sensitivity, high in accuracy, simple and rapid to operate, and suitable for rapid and large-scale screening anddetection of Seneca virus infected serum antibodies by veterinary departments and entry and exit inspection and epidemic prevention bureaus at all levels of grassroots.

Description

[0001] This application is a divisional application with the application number 201910548717.2, the application date is June 24, 2019, and the invention name is "a reagent for detecting Seneca virus antibodies and the polypeptide used therefor". technical field [0002] The invention relates to a polypeptide for detecting Seneca virus antibody and its application. Background technique [0003] Seneca virus disease is caused by Seneca virus type A of the Picornaviridae Senecavirus genus. It is a viral infectious disease that mainly infects pigs and is susceptible to pigs of different ages. In the initial stage of infection, adult pigs showed symptoms such as anorexia, lethargy, and fever, and then blisters occurred on the skin and mucous membranes of the nasal mirror, oral epithelium, tongue, and hoof crown, and then secondary ulcers and ulcers occurred. In severe cases, the hoof crown The ulcers in the hoof can spread to the bottom of the hoof, causing the hoof shell to loos...

AI Technical Summary

Problems solved by technology

The disease is mainly transmitted through contact, and can cause vesicular lesions on the snout and hooves of pigs. It is difficult to distinguish clinically from diseases such as foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis based on clinical symptoms.

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