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New application of palmitic acid as methylselenic acid anti-hepatoma sensitizer

A technology of methyl selenic acid and palmitic acid, applied in the field of medicine, can solve problems such as synergism, achieve synergistic anti-liver cancer treatment, significant application value, and enhance the effect of inducing apoptosis of liver cancer cells

Active Publication Date: 2021-01-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that palmitic acid has a synergistic effect on the apoptosis of cancer cells induced by methylselenoic acid

Method used

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  • New application of palmitic acid as methylselenic acid anti-hepatoma sensitizer
  • New application of palmitic acid as methylselenic acid anti-hepatoma sensitizer
  • New application of palmitic acid as methylselenic acid anti-hepatoma sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. The effect of palmitic acid on increasing the anti-liver cancer efficacy of methylselenic acid in mice.

[0025] Test method: 12 male BALB / C nude mice (Beijing Weitong Lihua Experimental Technology Co., Ltd.), 6-8 weeks old, 2 × 10 6 A HepG2 cell was mixed with an equal volume of Matrigel, injected subcutaneously into the right side of the mouse, and the tumor volume was measured with a vernier caliper. When the tumor volume is about 100-120mm 3 , the mice were randomly divided into control group, methyl selenic acid treatment group, palmitic acid treatment group and combined treatment group. The corresponding solvent was administered to the control group, the methyl selenic acid treatment group was intragastrically administered at a dose of 2 mg / kg per day, and the palmitic acid treatment group was intragastrically administered at a daily dose of 10.26 mg / kg. Mouse body weight and tumor volume were measured every other day. After the end of the experiment...

Embodiment 2

[0028] Embodiment 2, palmitic acid increases the apoptosis of HepG2 human liver cancer cells induced by methyl selenic acid

[0029] Human liver cancer cell line HepG2 cells were treated with MSeA at a low dose concentration of 2 μM for 24 hours, then 100 μM or 200 μM PA was added, and the number of apoptotic cells was detected by Annexin V-FITC / PI double staining method. Figure 4 The results showed that the number of cells treated with 2μMMSeA was not significantly different from that of the control group, the addition of PA induced the apoptosis of HepG2 cells, and the combined treatment group significantly caused cell death. The results showed that palmitic acid could increase the apoptosis of HepG2 human hepatoma cells induced by methylselenoic acid.

Embodiment 3

[0030] Example 3, methyl selenic acid prevents lipapoptosis of L02 human normal liver cells caused by palmitic acid

[0031] Using palmitic acid-treated L02 normal human liver cells as a model, the number of apoptotic cells was detected by crystal violet. After pretreatment of L02 cells with 2 μM MSeA for 24 h, corresponding concentrations of palmitic acid were added. Figure 5 The results shown indicated that the addition of MSeA significantly restored cell viability. The results showed that methyl selenic acid could prevent the apoptosis of L02 human normal liver cells induced by palmitic acid.

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Abstract

The invention provides a new application of palmitic acid as a methylselenic acid anti-hepatoma sensitizer. The invention provides a new application of palmitic acid and methylselenic acid in treatment of liver cancer. The invention discloses that palmitic acid has the effect of enhancing hepatoma cell apoptosis induced by methylselenic acid, has a good synergistic effect on anti-hepatoma treatment of methylselenic acid, and methylselenic acid has a good prevention effect on possible potential lipotoxicity of palmitic acid. The invention provides a scientific basis for developing anti-hepatomadrugs based on palmitic acid and methylselenic acid, and has important application value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the new use of palmitic acid as a methyl selenic acid anti-hepatoma sensitizer, that is, the new use of palmitic acid to enhance the anti-liver cancer effect of methyl selenic acid. Background technique [0002] Methylselenic acid (MSeA) is an organic compound of selenium, which can be directly reduced to methyl by glutathione and reduced nicotinamide adenine nucleotide phosphate (NADPH), which are widely present in tumor cells. Selenium has a strong anti-cancer effect. Methylselenoate induces a comprehensive redox reaction to modify proteins, and these changes are caused by intracellular stress caused by unfolded or misfolded proteins. [0003] Palmitic acid (PA) is a 16-carbon long-chain saturated fatty acid, which is the most abundant saturated fatty acid in the human body. Palmitic acid is not only an energy source, but also acts as a signaling molecule to regul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/20A61K31/185A61P35/00A61P1/16
CPCA61K31/185A61K31/20A61P1/16A61P35/00A61K2300/00
Inventor 赵冲张涵尹淑涛扈洪波
Owner CHINA AGRI UNIV
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