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Droplet single-layer tiled nucleic acid detection chip and preparation method thereof

A detection chip, tiled technology, used in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve the problems of volatility risk of fluorine organic substances, unavoidable pollution and demulsification, etc. Volatility risk problem, cost reduction, effect of reducing cost of use

Pending Publication Date: 2021-01-29
王晓冬
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the embodiments of the present invention is to provide a droplet single-layer tiled nucleic acid detection chip and its preparation method, which are used to solve the current micro-droplet nucleic acid detection technology, where the droplets need to be transferred multiple times, and it is difficult to avoid contamination during the transfer process. and demulsification, and when transferring droplets, there is a risk of volatility of fluoroorganic substances

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  • Droplet single-layer tiled nucleic acid detection chip and preparation method thereof
  • Droplet single-layer tiled nucleic acid detection chip and preparation method thereof

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Embodiment Construction

[0027] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiment of the application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiment of the application. Obviously, the described embodiment is only It is an embodiment of a part of the application, but not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0028] The purpose of the embodiments of the present invention is to realize the structure of a droplet single-layer tiled nucleic acid detection chip through a series of processes, so as to realize mass production. Specifically, the first is the construction and sealing of microchannels to realize the smooth generation of droplets; the second is the c...

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Abstract

The embodiment of the invention discloses a droplet single-layer tiled nucleic acid detection chip, a preparation method of the droplet single-layer tiled nucleic acid detection chip. According to thedroplet single-layer tiled nucleic acid detection chip, liquid droplets are collected and stored in a droplet tiled manner. After the liquid drops are collected, in-situ amplification and recognitionanalysis are performed, and the liquid drops are not transferred any more. Besides, mineral oil can be used, volatilization is not likely to happen in the PCR process at the room temperature, the cost of matched instruments is reduced, the use cost is effectively reduced, and the problem of the risk of volatilization of fluorine organic substances is fundamentally solved.

Description

technical field [0001] The invention relates to the technical field of manufacturing nucleic acid detection chips, in particular to a droplet single-layer tiled nucleic acid detection chip and a preparation method thereof. Background technique [0002] Since the polymerase chain reaction (Polymerase chain reaction, PCR) was proposed, nucleic acid amplification and quantification technology in vitro has developed into a core technology in the field of molecular biology. At present, PCR-based nucleic acid quantitative detection technology has become the main application technology for early diagnosis of diseases due to its remarkable advantages of fast analysis speed, high sensitivity, high throughput and short diagnosis time, and is widely used in molecular sequencing, gene expression Other research fields such as analysis, gene mutation study and drug screening. [0003] ABI Corporation of the United States has launched real-time fluorescent quantitative PCR (Real-time quan...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502784
Inventor 王晓冬
Owner 王晓冬