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Antibody pairs for use in a rapid influenza a diagnostic test

A type of influenza A and antibody pair technology, applied in disease diagnosis, measurement devices, peptide sources, etc., can solve problems such as unavailable combination

Pending Publication Date: 2021-02-05
GLAXOSMITHKLINE CONSUMER HEALTHCARE HLDG US
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The presence of the analyte renders the "Fab" binding domain unavailable for subsequent binding with additional reagents

Method used

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  • Antibody pairs for use in a rapid influenza a diagnostic test
  • Antibody pairs for use in a rapid influenza a diagnostic test
  • Antibody pairs for use in a rapid influenza a diagnostic test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1: Detection of Antibody Conjugate Dose Response to NP Antigens of H1N1 and H3N2 on Half (ie "Wet") Dip Tablets.

[0126] Capture antibody was striped onto 2.5 x 30 cm nitrocellulose membrane cards at a rate of 0.1 µl / sec using the Isoflow reagent dispensing module. The cards were dried overnight at ambient temperature in a humidity controlled drying room and then laminated with a 21 mm wide cellulose fiber core (222 Ahlstrom film). The cards were cut into 5 mm wide strips using a motion cutter (Kinematic Matrix 2360), resulting in 5 mm wide semi-lateral flow impregnated sheets ("semi-dipped sheets").

[0127] 20 μl each of the NP stock solution and the detection antibody-gold conjugate suspension were added to the 96-well plate. Place the half-dipped sample in the well and let stand until all the liquid is absorbed.

[0128] exist Figure 5 and 6 Shown above are the results of visual scoring of sample test line color intensity relative to a Rann scale of 0 ...

Embodiment 2

[0130] Example 2: Evaluation of antibody pairs (A) in "dry-up" format.

[0131] Nitrocellulose membranes were stripped with 1.0 mg / ml capture antibody and dried overnight at ambient temperature in a humidity-controlled environment. Spray the conjugate pad on the membrane card with detection antibody conjugate.

[0132] Using standard techniques, run the apparatus for 20 min with 60 μl of each H1N1 NP standard and detection antibody conjugate suspension.

[0133] Figure 7 The results of visually scoring the test lines relative to the Rann scale as previously described are shown in .

[0134] Antibody pair (A) has been shown to detect the NP antigen of the H1N1 strain to 1 ng / ml.

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Abstract

Novel antibody pairs for use in an Influenza A diagnostic test.

Description

[0001] Summary of the invention [0002] The present invention relates to a new selection of monoclonal antibody (mAb) classes that provide highly sensitive immunochromatographic assays (hereinafter "immunoassays") for the detection of human influenza A in biological samples. The mAb species are particularly useful when paired as detection and capture reagents in a "sandwich" lateral flow immunoassay (LFIA), such as in the so-called "rapid influenza diagnostic test" (RIDT) (also called is "proximity to the patient" or "point of care" test (POCT) or "dip tablet", see WHO paper, "Use of Influenza Rapid Diagnostic Tests" (2010)) commonly used. [0003] The novel mAb selections of the present invention, also referred to herein as "antibody (or mAb) pairs" or "matched antibody pairs" are selected from Affinity species, the viral nucleoprotein is a highly conserved protein across influenza A, which can be used to distinguish between influenza A and B, and has become a common target f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/08G01N33/558
CPCG01N2333/11G01N2800/52G01N33/56983G01N33/54388
Inventor D.J.彻奇A.尼古拉斯
Owner GLAXOSMITHKLINE CONSUMER HEALTHCARE HLDG US
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