A method for efficiently creating mstn gene mutants of Yellow River catfish

Abstract

The invention belongs to the field of fish genetics and breeding, and specifically discloses a method for efficiently creating mstn gene mutants of Yellow River catfish. This method is aimed at the Yellow River catfish Myostatin‑ Myostatin Gene Design Target Site, Synthetic gRNA, and Cas9 Proteins were co-microinjected into mature eggs of Yellow River catfish (stage V eggs), and then dry insemination was performed with yellow catfish sperm. After hatching and emergence, the mutation efficiency was tested. The knockout efficiency was 80%, and the average mutation efficiency was 74.1%. And the proportion of fully mutant individuals was 24%. Compared with the traditional fish gene editing method, this method not only obtains higher mutation efficiency, but also can efficiently obtain complete mutant individuals of Yellow River catfish, and the combination of gynogenesis increases the homozygosity of mutant individuals. For economic fish with a long reproductive cycle The rapid directional molecular design breeding of species also has important reference value.

Description

technical field [0001] The invention belongs to the field of fish genetics and breeding, and in particular relates to a method for efficiently creating mstn gene mutants of Yellow River catfish. Background technique [0002] Yellow River catfish (Silurus lanzhouensis), also known as Lanzhou catfish, is the name card of aquatic life in the Yellow River. Due to environmental damage and overfishing, the wild population of Yellow River catfish has shrunk sharply, and it has been included in the "Red List of Species in China". At the same time, due to the excellent characteristics of delicious meat, no intermuscular spines and high nutritional value, the Yellow River catfish has become an important economic fish, and the market demand is increasing year by year. However, it takes three years for the Yellow River catfish to reach sexual maturity, resulting in a relatively long genetic breeding cycle. Therefore, it is urgent to establish a set of directional, efficient and rapid ...

AI Technical Summary

Problems solved by technology

However, for economic fish such as the Yellow River catfish, the individual is large and the spawning plate is opaque, which limits the popularization and application to a certain extent.