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Breeding method of tetrastichus planipennisi and application of tetrastichus planipennisi to prevention and treatment of agrilus planipennis

A technique for P. chinensis and P. chinensis female bees, which are applied in the directions of application, botanical equipment and methods, monitoring of invasive species, etc., and can solve the problem of unrealized large-scale breeding of natural enemies, limited sample collection, and experimental research. and pest control efficiency

Active Publication Date: 2021-02-23
中国林业科学研究院森林生态环境与自然保护研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current domestic research on this natural enemy is only carried out after field collection (Gao Yu. Biological characteristics and host positioning of the scorpion beetle. Northeast Forestry University master's degree thesis, 2009.; Liu Zhenkai. Research on Bee Biology and Drought Tolerance. Master's Degree Thesis of Shenyang Agricultural University, 2011), this method is affected by various factors such as climate conditions, field environment, biological characteristics of insects and human activities, which is largely limited Improved sample collection, experimental studies, and pest control efficiency
Although some scholars have carried out indoor simple breeding of the aureus japonica collected in the wild, they are limited to studying the life history, biological characteristics and cold resistance of this natural enemy, and have not realized the large-scale breeding and application of this natural enemy. In the wild (Liu Zhenkai, 2011), it is of great significance to explore the indoor mass breeding and inter-forest release technology of the domestic A.

Method used

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  • Breeding method of tetrastichus planipennisi and application of tetrastichus planipennisi to prevention and treatment of agrilus planipennis
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  • Breeding method of tetrastichus planipennisi and application of tetrastichus planipennisi to prevention and treatment of agrilus planipennis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Sample wood species and host insects: the sample trees and hosts were respectively selected as follows: ash-white wax larvae, oak tree-queer larvae, walnut tree-walnut larvae, willow-golden beetle larvae , peach tree-pink-necked longhorn beetle larvae, respectively marked as treatment 1-5 (other conditions remain unchanged);

[0028] (2) Parallel experimental setup. Clean the surface of the sample wood that has just been felled with water and dry it. A small piece of bark is peeled off at different parts of the sample wood that are relatively flat and smooth. 3mm grooves, put healthy larvae into the grooves and cover with bark, then wrap them tightly with Parafilm to obtain worm-like wood with 10 host insects on each piece of wood, repeat 5 times;

[0029] (3) Insert the morphological lower end of the worm-like wood vertically into the wet flower mud, cover the outside with an insect raising tank, apply a little honey on the ventilation net on the side wall of the t...

Embodiment 2

[0037] (1) Wash and dry the surface of the just felled ash-like wood with water, peel off a small piece of bark at different parts of the sample wood that are relatively flat and smooth, and use a knife to make a 1cm wide and 3mm deep piece of bark In the groove, put the healthy 3-4 instar larvae of ash narrow gelatin into the groove, cover the bark, and then wrap it tightly with Parafilm to obtain a worm-like wood, with 10 host insects on each piece of sample wood, and repeat 5 times;

[0038] (2) Insert the morphological lower end of the worm-like wood vertically into the wet flower mud, cover the outside with an insect raising tank, apply a little honey on the ventilation net on the side wall of the tank, and set it aside. Afterwards, slightly open one part of the insect tank, and carefully put the healthy and mated female bee of the ash jellyfish into the tank. The ratio of the number of bees is 1:1, that is, 50 bees are received for each treatment;

[0039] (3) Parallel ...

Embodiment 3

[0046] (1) Wash and dry the surface of the just felled ash-like wood with water, peel off a small piece of bark at different parts of the sample wood that are relatively flat and smooth, and use a knife to make a 1cm wide and 3mm deep piece of bark In the groove, put the healthy 3-4 instar larvae of ash narrow gelatin into the groove, cover the bark, and then wrap it tightly with Parafilm to obtain a worm-like wood, with 10 host insects on each piece of sample wood, and repeat 5 times;

[0047] (2) Insert the morphological lower end of the worm-like wood vertically into the wet flower mud, cover the outside with an insect raising tank, apply a little honey on the ventilation net on the side wall of the tank, and set it aside. Afterwards, slightly open one part of the insect tank, and carefully put the healthy and mated female bee of the ash jellyfish into the tank. The ratio of the number of bees is 1:1, that is, 50 bees are received for each treatment;

[0048] (3) According...

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Abstract

The invention discloses a breeding method of tetrastichus planipennisi and application of the tetrastichus planipennisi to woodland prevention and treatment of agrilus planipennis. According to the method, agrilus planipennis larvae are used as hosts of the tetrastichus planipennisi, the tetrastichus planipennisi is bred by adopting an artificial inoculation method after proper conditions are screened, then natural enemies are used for preventing and treating the 3-4-year-old agrilus planipennis larvae, and the parasitic effect of the tetrastichus planipennisi is improved to the maximum extenton the premise that host specificity is stable.

Description

technical field [0001] The invention relates to an indoor breeding method of the parasitic natural enemy insect, the ash jelly beetle, and a technique for using the bee to carry out biological control of the ash jellyfish. Background technique [0002] Forest pests are a class of insects that are extremely destructive to forest ecosystems. According to incomplete statistics, the cost of their prevention and control in various countries has far exceeded hundreds of millions. In my country, the more serious forest pests currently mainly include leaf-eating pests such as the American white moth Hyphandria cunea and stem-boring pests such as longhorn beetles and jellyfish. Take the white wax narrow Jiding (Coleoptera: Chitinidae) as an example, this insect is a kind of stem-boring pest that mainly harms the trees of the genus Ash in the family Oleaceae, native to my country. In the early 1960s, the insect occurred in Northeast my country due to the introduction of white wax fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01G13/00A01M99/00
CPCA01K67/033A01G13/00A01M99/00A01M2200/012Y02A90/40
Inventor 王小艺党英侨魏可张彦龙曹亮明杨忠岐
Owner 中国林业科学研究院森林生态环境与自然保护研究所
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