A chalcone compound and its preparation method from buttercup thorn
A compound, chalcone technology, applied in the field of chalcone compound and its preparation from buttercup thorn, can solve the problem of chalcone products without separation, achieve high inhibitory activity and high extraction rate
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[0031] In a second aspect, the present invention provides a method for preparing the chalcone compound from Buttercup thorn, comprising:
[0032] Pulverize Buttercup thorn, extract with ethanol, and concentrate the extract to obtain the total extract;
[0033] The total extract was separated by silica gel column chromatography, and eluted by gradient hexane-ethyl acetate system to obtain 8 components in sequence;
[0034] The obtained third component was subjected to repeated silica gel chromatography in the n-hexane-ethyl acetate system to obtain the chalcone compound and the compound 4-methoxylonchocarpin, and the volume ratio of n-hexane to ethyl acetate was 1.8:8.2.
[0035] In some embodiments, the crushed Buttercup thorn has a particle size of 20-40 mesh.
[0036] In some embodiments, 3-4L of absolute ethanol is used for extraction per kilogram of Buttercup thorn.
[0037] Further, the extraction temperature with absolute ethanol is 20-35°C.
[0038] Furthermore, the ...
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[0042] Laboratory equipment
[0043] Nicolet-510P infrared spectrometer (USA), v max in cm -1 The unit is Bruker AMX-400 nuclear magnetic resonance instrument, TMS is the internal standard, the chemical shift is expressed in δ, and the coupling constant is expressed in J.
[0044] extraction and separation
[0045] 3.4kg of ranunculus thorns were crushed, leached with 10L ethanol at room temperature, the filtrate was filtered, and concentrated under reduced pressure to obtain 6.5g of total extract. The total extract was separated by silica gel column chromatography, and the gradient elution of n-hexane / ethyl acetate system was used. The gradient elution program was sequentially using n-hexane / ethyl acetate volume ratios of 10:90, 7.5:92.5, and 5:95 A mixed solvent was eluted to obtain 8 components (F1-8), wherein, component F3 (120 mg) was subjected to repeated silica gel chromatography (1.8:8.2, v / v) in the n-hexane / ethyl acetate system to obtain 4-benzyloxylonchocarpin (...
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