High-efficiency high-quality extraction method of ursolic acid in pomegranate

An extraction method, ursolic acid technology, applied in the direction of steroids, bulk chemical production, organic chemistry, etc., can solve the problems of weakening myocardial stress and conductivity, increasing cardiac output, increasing coronary blood vessels, etc., to achieve The effect of protecting the activity of substances, increasing the content, and high extraction rate

Active Publication Date: 2021-02-23
安徽硕佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lowering blood fat, lowering blood pressure, anti-atherosclerosis, can increase coronary blood flow, can improve coronary heart circulation, but coronary artery failure can be compensated, achieve heart-tonic effect, and can increase the ability of the myocardium to strengthen the heart. Sensitivity, increase cardiac output, weaken myocardial stress and conduction, have anti-ventricular fibrillation, cardiac fibrillation and volatile arrhythmia, etc. However, the current process of extracting ursolic acid from pomegranate peel is complicated and the yield is low. High, there are many shortcomings, therefore, improving the high-efficiency and high-quality extraction method of ursolic acid in a kind of pomegranate is the technical problem to be solved at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A high-efficiency and high-quality extraction method for ursolic acid in pomegranate, comprising the following steps:

[0020] The first step is to peel and deseed the pomegranate, collect the pomegranate peel and put it into a 45°C oven to dry to constant weight, then crush it with a pulverizer and pass through a 60-mesh sieve to obtain pomegranate peel powder. Transfer the pomegranate peel powder to a conical flask , add the extraction solvent to the conical flask, put the conical flask into the ultrasonic machine, at a temperature of 35 ° C and a power of 450 W, ultrasonically extract for 30 min, and then filter to obtain a crude extract 1;

[0021] Step 2: Put the crude extract 1 in a separatory funnel, add an equal volume of chloroform solution to the separatory funnel, fully shake and mix, and let stand for 2 hours at room temperature, then separate, collect the lower layer solution, and use rotary evaporation The instrument was dried to constant weight at a tempe...

Embodiment 2

[0027] A high-efficiency and high-quality extraction method for ursolic acid in pomegranate, comprising the following steps:

[0028]The first step is to peel and deseed the pomegranate, collect the pomegranate peel and put it in a 48°C oven to dry to constant weight, then crush it with a pulverizer and pass through a 60-mesh sieve to obtain pomegranate peel powder, which is transferred to a conical flask , add the extraction solvent to the conical flask, put the conical flask into the ultrasonic machine, at a temperature of 38 ° C and a power of 480 W, ultrasonically extract for 30 min, and then filter to obtain crude extract 1;

[0029] Step 2: Put the crude extract 1 in a separatory funnel, add an equal volume of chloroform solution to the separatory funnel, fully shake and mix, and let stand for 2 hours at room temperature, then separate, collect the lower layer solution, and use rotary evaporation The instrument was dried to constant weight at a temperature of 45 °C to ob...

Embodiment 3

[0035] A high-efficiency and high-quality extraction method for ursolic acid in pomegranate, comprising the following steps:

[0036] The first step is to peel and deseed the pomegranate, collect the pomegranate peel and put it in a 48°C oven to dry to constant weight, then crush it with a pulverizer and pass through a 60-mesh sieve to obtain pomegranate peel powder, which is transferred to a conical flask , add the extraction solvent to the conical flask, put the conical flask into the ultrasonic machine, at a temperature of 38 ° C and a power of 480 W, ultrasonically extract for 30 min, and then filter to obtain crude extract 1;

[0037] Step 2: Put the crude extract 1 in a separatory funnel, add an equal volume of chloroform solution to the separatory funnel, fully shake and mix, and let stand for 2 hours at room temperature, then separate, collect the lower layer solution, and use rotary evaporation The instrument was dried to constant weight at a temperature of 45 °C to o...

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Abstract

The invention discloses a high-efficiency high-quality extraction method of ursolic acid in pomegranate, which comprises the following steps: peeling pomegranate, collecting common waste pomegranate rind, drying, crushing, carrying out ultrasonic crushing, extracting an ursolic acid crude product in pomegranate rind by using [OMIM]Br ionic liquid as an extraction solvent through an ultrasonic method to obtain an ursolic acid crude product, then enriching through an anti-solvent precipitation crystallization method, carrying out de-esterification and de-coloring through petroleum ether, carrying out acid-base precipitation and impurity removal, and finally concentrating to obtain a high-quality ursolic acid finished product. According to the present invention, the method has advantages of simple extraction process, environmental protection, no harm to human body, high extraction rate, high ursolic acid purity and the like, and provides the basis for the further research and applicationof ursolic acid. According to the method, the [OMIM]Br ionic liquid is used as an extraction solvent, different from a typical organic solvent, the method has good stability, the defect that an additional possibly toxic organic solvent is used or a large amount of waste gas is generated is overcome, ultrasonic crushing extraction is used, and the activity of substances is protected.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to an efficient and high-quality extraction method of ursolic acid in pomegranate. Background technique [0002] Ursolic acid is a chemical product. The high content of ursolic acid is shiny prismatic or fine needle-like crystals, and the low content is brownish-yellow or yellow-green powder. It has a special smell and is a kind of natural plant. Pentacyclic triterpenoids. [0003] Pomegranate peel has a variety of functions such as astringent intestines and diarrhea, expelling roundworms and relieving pain, killing insects and relieving itching, astringing and relieving dysentery, etc. Pomegranate peel accounts for about 40% of the fruit weight. The pigment, ursolic acid, oleanolic acid, pectin, etc., ursolic acid, also known as ursolic acid, is a triterpenoid compound present in natural plants, which has sedative, anti-inflammatory, antibacterial, antidiabeti...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008Y02P20/54
Inventor 陈云帮吕钊彦余泽尧
Owner 安徽硕佳生物科技有限公司
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