Soybean sterile gene mutant, application and construction method of soybean sterile line

Abstract

The invention relates to the technical field of agricultural breeding, in particular to a soybean sterile gene mutant, application and a construction method of a soybean sterile line. The soybean sterile gene mutant is provided by the invention and comprises a mutant formed by mutation and / or deletion of a single base of a Glyma.13G114200 gene, the mutant is applied to construction or breeding ofthe soybean sterile line, a CRISPR-Cas9 gene editing tool is adopted to directly perform gene editing on wild soybeans to construct the soybean sterile line, or soybeans containing the gene mutant arehybridized with the wild soybeans, then offspring plants containing sterile genes are screened from offsprings by taking fertility as a screening standard or through a molecular marker, so that the sterile line of the soybeans can be quickly obtained or identified, and compared with the sterile line obtained by adopting a hybridization method, the breeding period is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a soybean sterile gene mutant, its application and a method for constructing a soybean sterile line. Background technique [0002] Soybean is an important grain and oil crop in the world, and it is also the main source of human high-quality protein and animal feed protein. Before the 1950s, China was the world's largest producer and exporter of soybeans. By 1996, my country had become a net importer of soybeans. At present, my country's soybean imports account for about 90% of China's soybean consumption market, which is affected by the international market. Seriously, therefore, it is imperative to develop domestically produced soybeans. However, due to low yields, poor comparative benefits, and weak farmers' willingness to plant, high subsidies are needed to ensure the development of the domestic soybean industry. [0003] Utilization of heterosis is one of the mo...

AI Technical Summary

Problems solved by technology

Before the 1950s, China was the world's largest producer and exporter of soybeans. By 1996, my country had become a net importer of soybeans. At present, my country's soybean imports account for about 90% of China's soybean consumption market, which is affected by the international market. Seriously, therefore, it is imperative to develop domestic soybeans, but due to low yields, poor comparative benefits, and farmers' unwillingness to plant, high subsidies are needed to ensure the development of the domestic soybean industry

[0013] It can be seen that the soybean sterile lines that are successfully commercially applied at present need to be obtained by crossing the cytoplasmic male sterile line. The breeding cycle is long and the stability is not strong. A faster and more efficient method for constructing soybean sterile lines is urgently needed.

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