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Three-dimensional reconstruction method of forest musk deer gland tissue

A three-dimensional reconstruction and organization technology, applied in the preparation of test samples, measurement devices, and material analysis through optical means, to achieve the effect of enriching the content of morphological structure

Pending Publication Date: 2021-02-23
四川养麝研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for three-dimensional reconstruction of the musk musk gland tissue, so as to solve the technical problem in the prior art that further in-depth research on the mechanism of musk musk secretion and the improvement of musk production cannot be carried out at the level of tissue and cell morphology

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  • Three-dimensional reconstruction method of forest musk deer gland tissue
  • Three-dimensional reconstruction method of forest musk deer gland tissue
  • Three-dimensional reconstruction method of forest musk deer gland tissue

Examples

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Effect test

Embodiment 1

[0052] A three-dimensional reconstruction method of musk deer gland tissue provided by the invention comprises the following steps:

[0053] (1) Sampling of isolated tissues: Immediately after the natural death of the male musk deer, the scent gland tissue was collected;

[0054] (2) Fixing: Immediately immerse the scent gland tissue obtained in step (1) in a 4% paraformaldehyde solution and store it in the dark for 24 hours to obtain a fixing material;

[0055] (3) Dehydration: use a dehydrator to dehydrate, take out the fixed material in step (2), trim the cut surface to make the cut surface smooth, and dehydrate the fixed material in 75% alcohol for 4 hours, 85% alcohol for 2 hours, and 90 Dehydration in % alcohol for 2 hours, dehydration in 95% alcohol for 1 hour, dehydration in 100% alcohol I for 30 minutes, dehydration in 100% alcohol II for 30 minutes to obtain dehydrated materials;

[0056] (4) Transparency: the dehydrated material obtained in step (3) is transparent ...

Embodiment 2

[0073] A three-dimensional reconstruction method of musk deer gland tissue provided by the invention comprises the following steps:

[0074] (1) Sampling of isolated tissues: Immediately after the natural death of the male musk deer, the scent gland tissue was collected;

[0075] (2) Fixing: Immediately immerse the scent gland tissue obtained in step (1) in 4% paraformaldehyde solution and store it in the dark for 48 hours to obtain the fixing material;

[0076] (3) Dehydration: use a dehydrator to dehydrate, take out the fixed material in step (2), trim the cut surface to make the cut surface smooth, and dehydrate the fixed material in 75% alcohol for 4 hours, 85% alcohol for 2 hours, and 90 Dehydration in % alcohol for 2 hours, dehydration in 95% alcohol for 1 hour, dehydration in 100% alcohol I for 30 minutes, dehydration in 100% alcohol II for 30 minutes to obtain dehydrated materials;

[0077] (4) Transparency: the dehydrated material obtained in step (3) is transparent ...

Embodiment 3

[0094] A three-dimensional reconstruction method of musk deer gland tissue, comprising the steps of:

[0095] (1) Sampling of isolated tissues: Immediately after the natural death of the male musk deer, the scent gland tissue was collected;

[0096] (2) Fixing: Immediately immerse the scent gland tissue obtained in step (1) in a 4% paraformaldehyde solution and store it in the dark for 36 hours to obtain a fixing material;

[0097] (3) Dehydration: use a dehydrator to dehydrate, take out the fixed material in step (2), trim the cut surface to make the cut surface smooth, and dehydrate the fixed material in 75% alcohol for 4 hours, 85% alcohol for 2 hours, and 90 Dehydration in % alcohol for 2 hours, dehydration in 95% alcohol for 1 hour, dehydration in 100% alcohol I for 30 minutes, dehydration in 100% alcohol II for 30 minutes to obtain dehydrated materials;

[0098] (4) Transparency: the dehydrated material obtained in step (3) is transparent in a mixed solution of absolute...

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Abstract

The invention provides a three-dimensional reconstruction method of a forest musk deer gland tissue and aims to solves the technical problems that a forest musk deer musk secretion mechanism cannot bedeveloped in the aspects of tissue and cellular morphological structure and the yield increase of musk cannot be further deeply researched in the prior art. The method comprises the steps of sampling, slicing, dyeing, scanning, three-dimensional reconstruction and the like of the forest musk deer gland tissue. According to the three-dimensional reconstruction method of the forest musk deer glandtissue, tissue slice dyeing is used for achieving in-vitro visualization of the musk gland tissue, and a good application effect is achieved; the physiological structure of the forest musk deer glandtissue is researched by the in-vitro visualization method of the forest musk deer gland tissue, and the three-dimensional in-vitro structure of the forest musk deer gland tissue is shown for the firsttime, so that the morphological structure content of the forest musk deer gland tissue is enriched, meanwhile, a morphological basis is provided for deeply researching the musk secretion reaction ofthe forest musk deer and improving the musk yield.

Description

technical field [0001] The invention relates to a method for three-dimensional reconstruction of musk musk gland tissue. Background technique [0002] Musk is a famous and precious Chinese medicine in my country. It has a medicinal history of more than 2,000 years and ranks first among the four major animal fragrances (musk, dragon birth incense, civet incense, and castoreum). It is warm in nature, pungent in taste, guixin, spleen meridian, has the effects of resuscitating the mind and refreshing the mind, promoting blood circulation and promoting menstrual flow, reducing swelling and relieving pain, etc. Closed, lumps, dystocia and stillbirth, chest obstruction and heartache, sudden pain in trusted subordinates, tumbling pain, arthralgia and numbness, carbuncle and scrofula, sore throat and other diseases. Among the 2621 prescriptions of Chinese patent medicines collected in China, there are 295 kinds that require musk. Among the 430 kinds of Chinese patent medicines prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N21/84
CPCG01N1/28G01N1/30G01N21/84
Inventor 郑程莉竭航赵贵军周磊雷美艳吴杰龚杭
Owner 四川养麝研究所
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