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Marker for predicting chemotherapy effect of cervical squamous carcinoma and screening method and application of marker

A technology for cervical squamous cell carcinoma and screening methods, applied in biochemical equipment and methods, measuring devices, biostatistics, etc., can solve problems such as uncontrollable expression of oncogenes and tumor suppressor genes

Pending Publication Date: 2021-03-09
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Genomic imbalance can lead to uncontrolled expression of oncogenes and tumor suppressor genes in cancer cells

Method used

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  • Marker for predicting chemotherapy effect of cervical squamous carcinoma and screening method and application of marker
  • Marker for predicting chemotherapy effect of cervical squamous carcinoma and screening method and application of marker
  • Marker for predicting chemotherapy effect of cervical squamous carcinoma and screening method and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Screening and Obtaining Differential Genes Related to Radiotherapy and Chemotherapy Effects of Locally Advanced Cervical Squamous Cell Carcinoma

[0050] 1. Search the GEO database for expression profile data sets related to radiotherapy and chemotherapy for locally advanced cervical squamous cell carcinoma. The search keywords are: "(locally advanced cervical squamous cell carcinoma) and (chemoradiotherapy OR radiotherapy OR radiation)", and the research types are limited to: expression profiling by array, restricted species: Homo sapiens. The selected data must meet the following conditions: (1) the data set must be the expression mRNA chip data of the entire cervical cancer genome; (2) the data must be a controlled study on the effectiveness and ineffectiveness of combined radiotherapy and chemotherapy for locally advanced cervical squamous cell carcinoma; (3) The data set case(responders)-control(non-responders) group must contain or exceed 3 samples; (4) ...

Embodiment 2

[0058] Example 2 predicts the transcription factor of the target gene

[0059] 1. Further use the NetworkAnalyst tool to predict the transcription factors that can regulate the expression of SRSF7, SNRPB, SNRPA, SF3A2 and PQBP1. We found that ZBTB7A, TFDP1 and ELF1 can regulate the expression of these five genes ( Figure 7 A).

[0060] 2. Correlation analysis showed that ELF1 was negatively correlated with SNRPB, SNRPA, and PQBP1 ( Figure 7 C).

[0061] 3. To find the ELF1 sequence, we used JASPAR analysis. From the sequence logo of ELF1, it can be seen that the sequence of ELF1 is higher in the 7-10 conservative type, and the frequency of G and A bases is very high ( Figure 7 D).

[0062] 4. We predicted the binding sequences of ELF1 and SNRPB, SNRPA and PQBP1 through JASPAR, and the results showed that ELF1 combined with the upstream of these genes. In this study, the 90% threshold was used for scoring, where the relative score of SNRPB was 0.9066, the relative scor...

Embodiment 3

[0063] Example 3 Verification of the correlation between target genes and curative effect of radiotherapy and chemotherapy for locally advanced cervical squamous cell carcinoma

[0064] 1. Differences in the expression levels of target genes in locally advanced cervical squamous cell carcinoma radiotherapy and chemotherapy sensitive and insensitive patients:

[0065] (1) Screen out the expression levels of target genes in three datasets (GSE56303-GPL10191, GSE56303-GPL16025, GSE56363);

[0066] (2) Divide the samples in the 3 data sets into the chemoradiotherapy-sensitive group and the insensitive progenitor;;

[0067] (3) For statistical analysis, the difference between the two groups of data was tested by t test, p-value0.5 were considered statistically significant.

[0068] (4) The expression level differences of target genes in locally advanced cervical squamous cell carcinoma sensitive to chemotherapy and insensitive patients are shown in Table 2:

[0069] Table 2 Diffe...

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Abstract

The invention discloses a marker for predicting the chemotherapy effect of cervical squamous carcinoma and a screening method and application of the marker. The marker is at least one of SRSF7, SNRPB,SNRPA, SF3A2 and PQBP1 genes. The invention also discloses application of a reagent for detecting the marker for predicting the chemotherapy effect of cervical squamous carcinoma in preparation of aproduct for predicting the cisplatin-based combined radiotherapy and chemotherapy effect of cervical squamous carcinoma. The reagent is used for detecting the frequency or expression level of the SRSF7, SNRPB, SNRPA, SF3A2 or PQBP1 genes. The genes screened by the invention can accurately distinguish patients with radiotherapy and chemotherapy responses from patients without radiotherapy and chemotherapy responses, and the patients can be assisted to select different treatment modes by utilizing the genes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a marker for predicting the curative effect of cervical squamous cell carcinoma chemotherapy, and the application of a reagent for detecting and predicting the curative effect of cervical squamous cell carcinoma chemotherapy in preparing a product for predicting the effect of combined chemotherapy for cervical squamous cell carcinoma. Background technique [0002] Cervical cancer is one of the leading causes of cancer death in women worldwide. Cervical cancer treatment remains a major challenge for researchers and physicians. In addition, mortality rates are significantly higher in less developed regions compared to industrialized countries, as most patients are diagnosed at an advanced stage. There are two types of cervical cancer, squamous cell carcinoma and adenocarcinoma, among which squamous cell carcinoma accounts for the largest proportion. At present, the treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574C12N15/11G16B15/30G16B20/30G16B40/20
CPCC12Q1/6886G01N33/57411G01N33/57484G16B15/30G16B20/30G16B40/20C12Q2600/106C12Q2600/118C12Q2600/158C12Q2600/166
Inventor 肖占刚杜富宽赵曰水沈晶马永顺张瑶吴旭李明星
Owner SOUTHWEST MEDICAL UNIVERISTY
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