Microbial agent for wheat planting
A microbial agent, wheat technology, applied in the field of agricultural microorganisms, can solve the problems of excessive use of chemical fertilizers, field management has not been considered, etc., to achieve the effect of increasing suberin, promoting early fruit maturity, and increasing production
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Embodiment 1
[0044] The preparation of embodiment 1 microbial bacterial agent
[0045] 1. Screening of functional strains
[0046] (1) Hydrogen oxidizing bacteria
[0047] Hydrogen oxidizing bacteria can use H 2 , with the participation of hydrogenase, CO assimilation 2 and H 2 Synthesize substances and perform chemoautotrophy. In the present invention, measure the H of strain with gas chromatography 2 Absorptive capacity. Purified Hydrogenophaga: Hydrogenophaga atypica, Hydrogenophaga palleronii, Hydrogenophaga flava; Pseudomonas: Pseudomonas Pseudomonas hydrogenovora, Rhodopseudomonas palustris; Rhodoparacoccus: Paracoccus sediminis and Paracoccus marcusii are inserted into the slope of R2A, and sealed after the bacterial lawn is present. The R2A slant inoculated with water was used as a control. Introduce a certain amount of H into the test tube 2 , fully mixed, in order to determine the aforementioned various hydrogen oxidizing bacteria on the H 2 Absorption, in a closed te...
Embodiment 2
[0064] Embodiment 2 measures the survival situation of bacteria in the microbial inoculum of the present invention
[0065] Soil conditions: pH7.55, ammonia nitrogen concentration 6.18mg / kg, nitrate nitrogen concentration 25.51mg / kg;
[0066] Seed soaking treatment: wheat Zhengmai 7698 was soaked in the microbial agent of Example 1 for four hours;
[0067] Spraying treatment: the leaves are sprayed every 6 weeks, and the spraying agent is the bacterial agent prepared in Example 1.
[0068] The specific operation method is as follows:
[0069] The soil from Xiping County, Henan Province was used to carry out a wheat pot experiment to determine the effect of the microbial agent of the present invention. Take by weighing 2 kg of soil in every pot, keep sufficient moisture in the pot, do not apply base fertilizer for potted plants, and do not use chemical drugs, directly sow three seeds of wheat Zhengmai 7698 per pot as a contrast, and soak the wheat seeds for four weeks in th...
Embodiment 3
[0073] Embodiment 3 The effect of bacterial agent of the present invention on the late-maturing phenomenon
[0074] The Zhengmai 7698 demonstration field was divided into a control group and an experimental group.
[0075] Control group 1: Wheat was sown in Yuange Village, Gaosi Town, Henan Province on October 11, 2019 by conventional methods. The basic properties of the soil were: pH 7.55, ammoniacal nitrogen concentration 6.18mg / kg, and nitrate nitrogen concentration 25.51mg / kg, apply compound fertilizer 64 kg per mu.
[0076] Control group 2: adopt the same method as control 1, the only difference is that compared with control group 1, the use of compound fertilizer is reduced, and the compound fertilizer is applied at 32 kg per mu.
[0077] Treatment group: sowed on October 11, 2019 in Yuange Village, Gaosi Town, Henan Province, soaked the winter wheat seeds with the bacterial agent of Example 1 for four hours before sowing, and then dried them on October 11, and appli...
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