Systems, devices, and methods for amplifying signals of a lateral flow assay
A lateral flow, fluid flow technology, applied in measurement devices, material analysis, instruments, etc. by observing the effect on chemical indicators, can solve the problem of inability to provide reliable or accurate indications of target analytes, and reduce false positives. Effects of negative readings
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Embodiment 1
[0084] Preparation of Lateral Flow Assays According to the Disclosure
[0085] The following examples describe the preparation of a lateral flow assay according to the present disclosure to measure analytes present in a sample at low concentrations. In this non-limiting example, the analyte of interest is influenza A virus ("Flu A").
[0086] A test strip is prepared having a conjugate pad with a sample receiving area and a buffer receiving area. The analyte-specific conjugate was prepared in solution and the solution was sprayed onto the sample receiving area by air jet deposition. Briefly, anti-Flu A antibody and biotin were incubated with gold nanoparticles to form analyte-specific conjugates. The analyte-specific conjugate was deposited on the conjugate pad at the sample receiving area in an amount of 7 μL / test strip by spraying the solution including the analyte-specific conjugate with an air jet. The conjugate pad is heated to dry the analyte-specific conjugate to t...
Embodiment 2
[0092] Amplification of signal from low concentration analytes
[0093] The following example illustrates the detection of low concentrations of FluA in samples using the lateral flow assay described in Example 1.
[0094] The lateral flow assay prepared in Example 1 was contacted with a sample containing a low concentration of Flu A. In this non-limiting example, Flu A antigen was a dilution of recombinant Flu A nucleoprotein expressed in E. coli and used for crude cell lysates. As a control, a lateral flow assay prepared as in Example 1 but lacking the signal-amplifying conjugate was used. Samples with Flu A were added to the sample wells of the lateral flow device and the control lateral flow device. Measure the resulting signal at various time points such as Figure 4 and shown in Table 1.
[0095] Table 1: Signal Variation Over Time
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[0098] Samples without the analyte of interest were also applied to lateral flow assays prepared with vari...
Embodiment
[0126] Features of Example Lateral Flow Devices
[0127] The lateral flow devices described herein include a device housing. The housing of any of the lateral flow devices described herein, including the top housing or the bottom housing, can be made from any suitable material, including, for example, vinyl, nylon, polyvinyl chloride, polypropylene, polystyrene , polyethylene, polycarbonate, polysulfone, polyester, urethane or epoxy. The housing can be prepared by any suitable method, including, for example, by injection molding, compression molding, transfer molding, blow molding, extrusion molding, foam molding, thermoforming, casting, layer deposition, or printing.
[0128]The lateral flow devices described herein may include sample wells in which a fluid sample is introduced to a test strip, such as, but not limited to, immunochromatographic test strips found in lateral flow devices. In one example, the sample can be introduced into the sample well by external applicat...
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