Method for optimizing loop-mediated isothermal amplification reaction

Abstract

The invention belongs to the technical field of biology and discloses a method for optimizing a loop-mediated isothermal amplification reaction. The method for optimizing the loop-mediated isothermalamplification reaction comprises the following steps: preparing an amplified substance for optimizing the loop-mediated isothermal amplification reaction from graphene quantum dots and carbon nanotubes; selecting a substance to be amplified according to amplification requirements, designing amplification primers through information on the substance to be amplified, meanwhile, setting a volume of the substance to be amplified and amplification conditions according to the captured information on the substance to be amplified, and then, establishing a loop-mediated isothermal amplification reaction system; and adding the amplified substance for optimizing the loop-mediated isothermal amplification reaction into the established loop-mediated isothermal amplification reaction system, and carrying out nucleic acid amplification, thereby preparing a nucleic acid reaction system. According to the method, the loop-mediated isothermal amplification reaction system can be optimized, the optimization effect on loop-mediated isothermal amplification is better, and meanwhile, the method is simple in operation and good in optimization effect and is applicable to molecule detection and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for optimizing loop-mediated isothermal amplification reaction. Background technique [0002] Currently: Loop-mediated isothermal amplification (LAMP for short) is a technology for nucleic acid amplification under isothermal conditions, which can be widely used in food safety testing and other fields. This technique has high sensitivity, and it can produce 109-1010 times amplicons under isothermal conditions for 15min-1h amplification. If the experimental environment is polluted by aerosols, it is easy to produce false positive results. In addition, since multiple primers are involved in the amplification reaction process, non-specific binding between primers is easy to produce primer dimers, thereby consuming the reaction substrate in the reaction system, reducing the reaction efficiency and detection sensitivity, and at the same time easily leading to negative results. Fa...

AI Technical Summary

Problems solved by technology

However, in the prior art, the optimization scheme of graphene quantum dots for ring-mediated isothermal amplification reaction has poor optimization effect on ring-mediated isothermal amplification, and the operation is complicated

[0004] Through the above analysis, the existing problems and defects of the prior art are: in the prior art, the scheme for optimizing the graphene quantum dots for the ring-mediated isothermal amplification reaction has a poor optimization effect on the ring-mediated isothermal amplification, and Complicated operation

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