Application of kaempferol in preparation of health food or medicine for preventing blue light damage
A technology for health food and blue light damage, which is applied to the functions, applications, and food sciences of food ingredients. It can solve the problems of undiscovered protection against blue light damage, and achieve the effect of improving high temperature stress ability and behavioral activities
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Embodiment 1
[0033] Example 1: Construction of Blu-ray culture environment
[0034] Instrument: LED patch light strip (Taole Anju home lighting series)
[0035] Method: Measure the wavelength range of the LED light band, and select the best LED light source with a narrower and purer band. For this experiment, we used a type 120B blue light strip with a wavelength range of 457±30nm (90% of the total irradiance). Turn off the original light source of the incubator, and put the above-mentioned LED light strip into the incubator as the only light source. The distance from the light source is different, and the light intensity is different. Measure the light intensity with an illuminance meter, determine the position where the light intensity is 2500 lux, and put the fruit fly tube into the above position. At the same time, cardboard is used to resist the influence of the light source from the side to ensure the accuracy of the data.
[0036] For wavelength measurements of blue light, see ...
Embodiment 2
[0037] Example 2: Effect of kaempferol on the life cycle of Drosophila exposed to blue light
[0038] Methods: Set up the administration group and the blank group. Control group: Add 50ul of absolute ethanol to 1.0× low-calorie medium. Experimental group: 50ul of the above three concentrations of kaempferol reagents were added to 1.0× low-calorie medium. 3 tubes per group, 40 flies per tube. After every 2 days, the surviving fruit flies were transferred to the next new medium with the same conditions, and the number of dead fruit flies was counted until all died.
[0039] The experimental results are shown in Table 1:
[0040] Table 1: Effect of kaempferol on the lifespan of Drosophila exposed to 2500lux blue light
[0041]
[0042] It can be seen from Table 1 that in 1.0× medium, kaempferol has an effect on Drosophila exposed to blue light,
[0043] However, the concentration is different and the degree of effect is also different. For red-eyed female flies, 10 -1 T...
Embodiment 3
[0045] Embodiment 3: 37 degree high temperature stress experiment
[0046] To detect the change of kaempferol in the survival rate of Drosophila exposed to blue light under the condition of high temperature stress.
[0047] Instrument: transparent water bath (Jintan Experimental Instruments, China)
[0048] Method: Cultivate under 2500lux blue light environment until the 8th day, put the fruit flies into empty bottles, plug them with cotton wool, place them in a water bath at 37 degrees, and record the number of dead fruit flies every 10 minutes (death is defined as observation 10 seconds without any activity), until the end. The experiment should be repeated 5 times in parallel, with 10 flies in each group.
[0049] See the experimental results figure 2 . Depend on figure 2 It can be seen that feeding kaempferol to Drosophila exposed to blue light can improve their stress ability. For red-eyed female flies, 10 -1 mol / L kaempferol can prolong the average lifespan of D...
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