Cell traction measurement method and system based on Fourier transform, and medium

Abstract

The invention relates to a cell traction measurement method and system based on Fourier transform and a medium. The method comprises the following steps: acquiring a high-magnification microscopic image of a cell region which has a good growth state and in which cells stretch a microcolumn; converting the high-power microscopic image into a grey-scale image; performing two-dimensional fast Fouriertransform on the grey-scale map to obtain a spectrogram; performing filtering and inverse Fourier transform on a certain single frequency point in the spectrogram to obtain an amplitude distributiondiagram of the micro-column array; and converting the amplitude distribution diagram into an offset distribution diagram according to the corresponding relationship between the offset value and the amplitude, and calculating according to the rigidity of PDMS to obtain a final cell force distribution diagram. The distribution condition of the cell traction force can be obtained more intuitively andmore quickly. The invention can be widely applied to the technical field of digital image processing.

Description

technical field [0001] The invention relates to the technical field of digital image processing, in particular to a method, system and medium for measuring cell traction force based on Fourier transform. Background technique [0002] Life has been living in and adapting to the mechanical environment since its birth. The mechanical interaction between cells and the extracellular microenvironment plays a crucial role in cell migration, proliferation and differentiation. However, due to the inherent biological complexity of cells and the constraints of the micro-nano scale, traditional macro-experimental mechanical methods and techniques are often difficult to use directly. The vertically aligned polydimethylsiloxane (ploydimethylsiloxane, PDMS) prepared by microfabrication technology can support cell adhesion after the top surface is modified, and the traction force generated after cell adhesion will cause the microcolumn to bend, and the measured The traction force distribu...

AI Technical Summary

Problems solved by technology

However, when using the centroid method to process PDMS microcolumn images under a high-power microscope, it may be difficult to select a suitable threshold when processing the image due to the uneven base of the microcolumn array itself or uneven illumination, which will affect the subsequent series of operations; and the centroid The image processing method requires the original position as a standard, and the shooting of the original position or the processing of the algorithm is relatively cumbersome; in addition, the process of binarization of the centroid method causes the contour to jump greatly, which will bring errors to the centroid positioning of the micro-pillar end face

Therefore, although the centroid method is faster than the Hough transform method, due to the limitations of this method, there are still difficulties in choosing a reasonable threshold when the illumination is uneven, the original position is required as a standard, and the process of binarization These problems make the contour jump big

Images