The invention specifically relates to a communication method of a circuit board and the circuit board, and a host computer, a slave computer and microcontrollers are provided. The host computer and the slave computer communicate with each other via a connecting line, the microcontrollers respectively communicate with the host computer and the slave computer via serial communication ports. The method includes the steps of powering up the host computer and the slave computer, sending a synchronous code from the host computer to the slave computer, the searching the corresponding rate parameters from the slave computer's storage rate parameter list according to the synchronous code received, and adjusting to the corresponding execution value and storing the execution value in the memory of the slave computer, executing the execution value after adjustment and sending the synchronous code to the host computer by the slave computer, and receiving and storing the synchronous code send by the slave computer and realizing the communication between the host computer and the slave computer by the host computer. The invention has the advantages that: 1. simplifying the communication by connecting the host computer, the slave computer and the microcontrollers and reducing the fault caused by connection; 2. realizing a unified connection interface which is compatible with different product interfaces, reducing computer types, giving convenience for maintenance, and lowering the cost.