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Method for producing butyric acid by fermenting corn starch

A technology of corn starch and production method, applied in the direction of fermentation and the like, can solve the problems of high cost and high cost of butyric acid, and achieve the effects of increasing the fermentation capacity, reducing the cost of using raw materials, and reducing the cost of equipment

Pending Publication Date: 2021-04-09
ZHUMADIAN HUAZHONG CHIA TAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The carbon source and nitrogen source in the existing fermentation medium formula can basically meet the growth needs of butyric acid fermentation bacteria, but the cost of producing butyric acid by using glucose fermentation as a carbon source is relatively high, and glucose needs to be eliminated separately and then combined. , so the increased cost is more

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for producing butyric acid by fermenting cornstarch, comprising the following steps: the fermentation production method includes seed cultivation and fermentation cultivation, the butyric acid fermentation bacteria are inoculated in the seed tank culture medium for seed cultivation, glucose 20g / L, yeast extract 2g / L, peptone 4g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H 2 O 0.02g / L, ZnSO 4 ·7H 2 O 0.002g / L, the balance is water. Then inoculated in the fermenter medium for fermentation culture, the fermenter medium composition includes: glucose 20~30g / L, cornstarch 25~30g / L, amylase 0.004g / L, organic nitrogen source 2~8g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H 2 O 0.02g / L, ZnSO 4 ·7H...

Embodiment 2

[0015] A method for producing butyric acid by fermenting cornstarch, comprising the following steps: the fermentation production method includes seed cultivation and fermentation cultivation, the butyric acid fermentation bacteria are inoculated in the seed tank culture medium for seed cultivation, glucose 20g / L, yeast extract 2g / L, peptone 4g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H 2 O 0.02g / L, ZnSO 4 ·7H 2 O 0.002g / L, the balance is water. Then inoculated in the fermenter medium for fermentation culture, the fermenter medium composition includes: glucose 10~12g / L, cornstarch 40~58g / L, amylase 0.006g / L, organic nitrogen source 2~8g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H 2 O 0.02g / L, ZnSO 4 ·7H...

Embodiment 3

[0017] A method for producing butyric acid by fermenting cornstarch, comprising the following steps: the fermentation production method includes seed cultivation and fermentation cultivation, the butyric acid fermentation bacteria are inoculated in the seed tank culture medium for seed cultivation, glucose 20g / L, yeast extract 2g / L, peptone 4g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O 0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H2 O 0.02g / L, ZnSO 4 ·7H 2 O 0.002g / L, the balance is water. Then inoculate in fermentor medium and carry out fermentation culture, and fermentor medium composition comprises: cornstarch 50~60g / L, amylase 0.01g / L, organic nitrogen source 2~8g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 2H 2 O0.015g / L, MnSO 4 ·H 2 O 0.01g / L, CoCl 2 ·6H 2 O 0.02g / L, ZnSO 4 ·7H 2 O 0.002g / L, t...

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PUM

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Abstract

The invention discloses a method for producing butyric acid by fermenting corn starch. The method comprises the following steps: a fermentation production method comprises seed culture and fermentation culture; butyric acid fermentation thalli are inoculated into a seed tank culture medium for seed culture; then, the butyric acid fermentation thalli are inoculated into a fermentation tank culture medium for fermentation culture; the fermentation tank culture medium comprises 25-60 g / L of corn starch, 0.004-0.01 g / L of amylase, 2-8 g / L of an organic nitrogen source, 2 g / L of (NH<4>)<2>SO<4>, 1 g / L of K<2>HPO<4>, 1 g / L of KH<2>PO<4>, 0.1 g / L of MgSO<4>.7H<2>O, 0.015 g / L of FeSO<4>.7H<2>O, 0.015 g / L of CaCl<2>.2H<2>O, 0.01 g / L of MnSO<4>.H<2>O, 0.02 g / L of CoCl<2>.6H<2>O, 0.002 g / L of ZnSO<4>.7H<2>O, and the balance of water. According to the method for producing butyric acid by fermenting corn starch provided by the invention, the quick-acting carbon source glucose in the fermentation culture medium is partially and completely replaced by the slow-acting carbon source corn starch (or corn flour) on the basis of the culture medium, so that the use cost of raw materials is reduced; the problems of equipment cost, labour cost, power consumption cost and the like caused by independent sterilization of glucose in the fermentation process are reduced; and the fermentation productivity is improved.

Description

technical field [0001] The invention belongs to the technical field of butyric acid fermentation, in particular to a method for producing butyric acid by fermenting cornstarch. Background technique [0002] Butyric acid is a platform compound widely used in food, chemical, pharmaceutical and other industries, with an annual global market demand of about 80,000 tons. At present, there are two main ways to produce butyric acid: chemical synthesis and microbial fermentation. The chemical synthesis method has high output and good product specificity, but the production operation is carried out under high temperature and high pressure conditions, and non-renewable fossil raw materials are used. Butyric acid can also be produced by microbial fermentation, because the use of biomass raw materials is carbon-neutral. Compared with chemical synthesis and fermentation, it has great advantages in mitigating global warming and greenhouse effect and using renewable resources. [0003] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/52
CPCC12P7/52
Inventor 李冰吕向云曹华伟娄百勇李学成杨永
Owner ZHUMADIAN HUAZHONG CHIA TAI