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16S and metagenome sequencing data association analysis method and system, and equipment

A technology of association analysis and metagenomic, applied in the field of data processing and analysis, to achieve the effect of complementing each other

Active Publication Date: 2021-04-16
GUANGZHOU GENE DENOVO BIOTECH
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Problems solved by technology

[0004] In order to overcome the problems existing in related technologies, the present invention provides a method, system and equipment for correlation analysis of 16S and metagenomic sequencing data, so as to solve the problem of single 16S sequencing data and single Pitfalls in Metagenome Sequencing Data Analysis

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[0070] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention.

[0071] In some processes described in the specification and claims of the present invention and the above-mentioned drawings, a plurality of operations appearing in a specific order are contained, but it should be clearly understood that these operations may not be performed in the order in which they appear herein Execution or parallel execution, the serial numbers of the operations, such as 101, 102, etc., are only used to distinguish different operations, and the serial numbers themselves do not represent any execution order. Additionally, these processes can include more or fewer operations, and these operations can be performed sequentially or in parallel. It should be n...

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Abstract

The invention relates to a 16S and metagenome sequencing data association analysis method and system, and equipment. According to the scheme, the method comprises the following steps: performing 16S sequencing and metagenomics sequencing on a sample to obtain 16S omics data and metagenomics data; carrying out microPITA analysis according to the 16S omics data to obtain a representative sample; performing Ween analysis according to the 16S omics data and the metagenomics data to obtain common and specific statistical species / function statistics, and performing graphical display; carrying out species / function distribution analysis to obtain species / function abundance changes and main species / function types in all the samples of the two omics; and performing correlation analysis and environmental factor correlation analysis according to the 16S omics data and the metagenomics data. According to the scheme, the 16S and metagenome sequencing data correlation analysis method is provided, the metagenome extension data is used for researching the depth, 16S is used for verifying the analysis accuracy of metagenome data, and mutual complementation and verification of two omics data are achieved.

Description

technical field [0001] The present invention relates to the field of data processing and analysis, in particular to a method, system and equipment for correlation analysis of 16S and metagenomic sequencing data. Background technique [0002] Microorganisms are closely related to biology, environment, production and other aspects, and play an important role. Among them, human microorganisms are considered as the second genome of human beings. With the development of science and technology, high-throughput sequencing is also becoming more and more popular. 16S sequencing technology and metagenomic sequencing technology are two important high-throughput sequencing methods. 16S sequencing technology generally only focuses on the study of community diversity changes, while metagenomic sequencing focuses on mining the functional structure of microbial communities. [0003] The existing technology has the following defects: although the 16S sequencing data can be used to analyze ...

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Application Information

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IPC IPC(8): G16B10/00G16B30/00G16B35/20G16B45/00G16B50/00
Inventor 高川周煌凯陈飞钦艾鹏张秋雪
Owner GUANGZHOU GENE DENOVO BIOTECH
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