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Electrochemical sensing analysis method of environmental hormone p-nitrophenol

An environmental hormone and analysis method technology, applied in the field of electrochemical sensing and analysis of environmental hormone p-nitrophenol, can solve the problems of lack of surface active sites, serious carrier recombination, small specific surface area, etc., to improve electrocatalysis Speed, low cost, easy to synthesize effects

Active Publication Date: 2021-04-23
WUHAN INSTITUTE OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to its wide band gap, severe carrier recombination, lack of surface active sites, thick shee

Method used

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  • Electrochemical sensing analysis method of environmental hormone p-nitrophenol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] WO 2.9 / g -C 3 N 4 Preparation of stepped heterojunction:

[0030] Weigh 2-10g of melamine into a ceramic crucible, heat and stir evenly, put it into a muffle furnace for heating, the heating rate is 3-12°C / min, the heating temperature is 500-600°C, the constant temperature is 3-10h, and it is ready after cooling Pale yellow g-C 3 N 4 powder. Then g-C 3 N 4 Put it in a cell wall breaker for ultrasound, the ultrasound power is 800-1500W, the ultrasound start: stop time ratio is 10:2, and the ultrasound time is 2-4h. Take 2-10mL of supernatant and dilute to 0.5-1.5L, sonicate at 80-140W for 1-4h, and let it rest overnight to obtain a thin layer of g-C 3 N 4 Dispersion liquid; 0.05~0.20g of WCl 6 Put it in 20-50mL of absolute ethanol, and ultrasonically dissolve it completely. The ultrasonic power is 80-140W, and the ultrasonic time is 3-10min. In the still, seal and heat to 150-200°C for 10-15h. After the reaction is over, cool down to room temperature naturall...

Embodiment 2

[0035] Equipped with 0.04 ~ 100μM environmental hormone 4-NP sample of known concentration, the WO 2.9 / g -C 3 N 4 The modified electrode performs DPV scanning in the environmental hormone 4-NP, and calculates the linear equation according to the magnitude of the oxidation peak current and the corresponding environmental hormone 4-NP concentration: I p =0.0491c+0.0908(R 2 =0.9985); c is the concentration (μmol / L) of environmental hormone 4-NP, I p is the oxidation peak current (μA). According to this equation, the unknown concentration of environmental hormone 4-NP can be quantitatively detected.

Embodiment 3

[0037] With reference to example 1, prepare WO 2.9 / g -C 3 N 4 Modify the electrode, put the modified electrode into the sodium acetate-acetate buffer solution containing the analyte at a pH of 3 to 7 for DPV scanning, and measure the current intensity corresponding to the electrocatalytic oxidation peak of the environmental hormone 4-NP to determine the environmental hormone 4 - NP content. Its optimal pH value is 5, and the peak current is the largest. According to the relationship between pH and voltage, the number of electrons and protons transferred is analyzed, and E is obtained by linear regression analysis of pH value and voltage. pa (V)=-0.06019pH+1.376 (R 2 =0.993), according to the formula: d Epc / d pH =0.059m / n, where m is the number of protons, n is the number of electrons, and dE pc / d pH =0.06019, close to 0.059, so it can be concluded that m / n=1, that is, in WO 2.9 / g -C 3 N 4 On the electrode, the number of electrons transferred during the electroca...

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Abstract

The invention discloses an electrochemical sensing analysis method of environmental hormone 4-NP, which comprises the steps of ultrasonically dispersing WO2.9/g-C3N4 stepped heterojunction in deionized water to obtain a dispersion liquid with the concentration of 0.0002-0.01 g/mL; adding 1-10 [mu] L of the WO2.9/g-C3N4 dispersion liquid to a clean impurity-free bare glassy carbon electrode for modification, and then baking the glassy carbon electrode under an infrared lamp for 3-10 min to obtain a modified electrode; putting the modified electrode into to-be-detected liquid to carry out a DPV test; and if an obvious oxidation peak appears in the range of 0.8-1.2V, carrying out qualitative detection on the liquid to be detected. When the concentration is in a range of 0.4-100 [mu] mol/L, the oxidation peak current value and the concentration are in a good linear relationship, the detection limit is 0.13 [mu] mol/L, and the method has good stability and reproducibility, and can be applied to detection of environmental hormone 4-NP in an actual water sample.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensing, and in particular relates to an electrochemical sensing analysis method for environmental hormone p-nitrophenol. Background technique [0002] Environmental hormones refer to chemical substances that exogenously interfere with the endocrine of organisms. These substances are similar to hormones and bind to hormone receptors in the human body, affecting the amount of original hormones in the organism, causing normal hormone imbalance, interfering with the metabolic balance in the organism, and then affecting normal physiological functions of living organisms. Among them, phenolic environmental hormones have received the most attention. With the development of industrialization and the intensification of environmental pollution, the residues of phenolic environmental hormones in the environment are increasing day by day. They have mutagenic, cytotoxic and phytotoxic effects, and are...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/3277G01N27/3278G01N27/308G01N27/48
Inventor 江吉周何小苗邹菁白赛帅
Owner WUHAN INSTITUTE OF TECHNOLOGY
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